Title

Determining which Domain of Iqg1 Binds to Formins during Cytokinesis

Presenter Information

Brandon Drennen

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Shannon, Katie

Advisor's Department

Biological Sciences

Funding Source

Opportunities for Undergraduate Research (OURE)

Abstract

The final step in cell division is cytokinesis. Cytokinesis must be coordinated with mitosis in order to prevent aneuploidy. Budding yeast are a good model organism for studying cytokinesis due to genetic and molecular tools and inexpensive growth medium. Iqg1 is a protein that has been shown to regulate cytokinesis in budding yeast cells. The Iqg1 protein contains four domains: IQ motifs that are needed for localization of Iqg1 to the contractile ring, a CHD domain required for actin recruitment, a GAP domain essential for contraction, and a RGCT domain that was predicted to interact with formins Bni1 and Bnr1. However, we found that a deletion of the RGCT domain of the protein is still able to interact with the formins and is required for cell viability. Our current goal is to determine domain of the protein that is responsible for formin binding using different mutant strains.

Biography

Brandon Drennen is from the small town of Freeburg in Missouri. He has been attending the Missouri University of Science and Technology for 4 years now and will graduate this May. After graduation he will be moving to Baltimore, MD to attend grad school and work on his PhD in Pharmaceutical Sciences.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hall

Presentation Date

16 Apr 2014, 9:00 am - 11:45 am

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Apr 16th, 9:00 AM Apr 16th, 11:45 AM

Determining which Domain of Iqg1 Binds to Formins during Cytokinesis

Upper Atrium/Hall

The final step in cell division is cytokinesis. Cytokinesis must be coordinated with mitosis in order to prevent aneuploidy. Budding yeast are a good model organism for studying cytokinesis due to genetic and molecular tools and inexpensive growth medium. Iqg1 is a protein that has been shown to regulate cytokinesis in budding yeast cells. The Iqg1 protein contains four domains: IQ motifs that are needed for localization of Iqg1 to the contractile ring, a CHD domain required for actin recruitment, a GAP domain essential for contraction, and a RGCT domain that was predicted to interact with formins Bni1 and Bnr1. However, we found that a deletion of the RGCT domain of the protein is still able to interact with the formins and is required for cell viability. Our current goal is to determine domain of the protein that is responsible for formin binding using different mutant strains.