Title

The Effects of Gallein on M3 Muscarinic Receptors

Presenter Information

Alex Willis

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Aronstam, Robert

Advisor's Department

Biological Sciences

Funding Source

cDNA Resource Center

Abstract

Gallein is a large cyclic carbon molecule with multiple functional groups. With no known medical use and few research articles, the only known action is that the drug has been found to effect G-protein subunits, specifically the GBγ subunit expressed in native rat olfactory receptors. I determined the effects of Gallein on calcium signaling underlying transmission mediated by human M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Receptor binding was determined in radiolabelled ligand binding assays; changes in intracellular calcium concentrations were determined using a fura-2 ratiometric imaging protocol. Gallein had a distinct inhibitory effect on store operated calcium (SOCE) that was induced by activation of the M3 receptors. No inhibition of IP3 receptor mediated calcium release was seen. With more testing, Gallein might prove to have a pharmacological benefit.

Biography

Alex is a junior in Biology. While getting his B.A., he is also pursuing a minor in Chemistry and Spanish. He is the Public Relations Officer for the pre-health organization, Scrubs. He has hopes to go to medical school after his senior year and become a M.D. in Ophthalmology.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hallway

Presentation Date

10 Apr 2012, 9:00 am - 11:45 am

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Apr 10th, 9:00 AM Apr 10th, 11:45 AM

The Effects of Gallein on M3 Muscarinic Receptors

Upper Atrium/Hallway

Gallein is a large cyclic carbon molecule with multiple functional groups. With no known medical use and few research articles, the only known action is that the drug has been found to effect G-protein subunits, specifically the GBγ subunit expressed in native rat olfactory receptors. I determined the effects of Gallein on calcium signaling underlying transmission mediated by human M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Receptor binding was determined in radiolabelled ligand binding assays; changes in intracellular calcium concentrations were determined using a fura-2 ratiometric imaging protocol. Gallein had a distinct inhibitory effect on store operated calcium (SOCE) that was induced by activation of the M3 receptors. No inhibition of IP3 receptor mediated calcium release was seen. With more testing, Gallein might prove to have a pharmacological benefit.