Title

Effects of Nitric Oxide on Store Operated Calcium Entry

Presenter Information

Katie Payne

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Aronstam, Robert

Advisor's Department

Biological Sciences

Funding Source

Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program

Abstract

S-nitrosoglutathion (SNG) is a well known smooth muscle relaxant and a nitric oxide donor when introduced into a cell. SNG has a very short half life so it must be synthesized before every experiment. It was made by using Glutathione (GSH) and Sodium Nitrate (NaNO2). We determined the effects of nitric oxide on calcium signaling by utilizing M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Cytotoxicity was determined using a dye reduction assay. Nitric oxide was found to have an EC50 at 10mM, and had an inhibitory effect on store operated calcium entry (SOCE). Cytosolic calcium concentrations were measured using a calcium sensitive fluorescent dye (fura-2AM) and a ratiometric imaging program.

Biography

Katie is in her second year at Missouri S&T. She is from Steelville, MO and plans to attend a medical school after receiving her undergraduate degree in Biological Sciences with a minor in Chemistry and Theatre. Katie Payne is active in Alpha Psi Omega, the National Theatre Honors society. Katie Payne works as a research assistant at Dr. Aronstam’s cDNA lab and she volunteers at the PCRMC Emergency Department in her free time.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hallway

Presentation Date

10 Apr 2012, 9:00 am - 11:45 am

Comments

Joint project with Megan Koerner

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Apr 10th, 9:00 AM Apr 10th, 11:45 AM

Effects of Nitric Oxide on Store Operated Calcium Entry

Upper Atrium/Hallway

S-nitrosoglutathion (SNG) is a well known smooth muscle relaxant and a nitric oxide donor when introduced into a cell. SNG has a very short half life so it must be synthesized before every experiment. It was made by using Glutathione (GSH) and Sodium Nitrate (NaNO2). We determined the effects of nitric oxide on calcium signaling by utilizing M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Cytotoxicity was determined using a dye reduction assay. Nitric oxide was found to have an EC50 at 10mM, and had an inhibitory effect on store operated calcium entry (SOCE). Cytosolic calcium concentrations were measured using a calcium sensitive fluorescent dye (fura-2AM) and a ratiometric imaging program.