Title

Comparison of Potency of SOCE Inhibition by Naturally Occurring Biphenolic Compounds

Presenter Information

Alexis Martin

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Aronstam, Robert

Advisor's Department

Biological Sciences

Funding Source

cDNA Resource Center

Abstract

Asian herbal medicine has used plants to treat many diseases for years. Two main compounds derived from Magnolia trees, honokiol and magnolol, have become accepted in their treatment of mental disorders such as anxiety and depression. These compounds have more recently been studied for their unique abilities to induce apoptosis and cytotoxicity in cancer cell lines. The purpose of this study was to determine and compare how honokiol, its sister compound, magnolol, and two other isomers, compound 24 and 27, affect intracellular calcium concentrations in cells expressing muscarinic receptors. Calcium concentrations were determined using a Fura-2 ratiometric imaging protocol. It was determined that all four compounds inhibit SOCE, an ER calcium-replenishing mechanism, known to play a crucial role in apoptosis and cell cycle regulation. Of this, compound 27 was found to be the most potent. It is recommended that the isomers of honokiol be specifically looked at for their anti-tumorigenic properties.

Biography

Alexis is a senior of the Biological Science’s department. She has been a research assistant in the cDNA Resource Center for two and a half years. Alexis is an active member of the Biological Honor’s society, Phi Sigma, and secretary of a women’s service organization, Lambda Sigma Pi. She will be attending medical school at Kansas City University of Medicine and Biosciences in the fall.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hallway

Presentation Date

10 Apr 2012, 9:00 am - 11:45 am

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Apr 10th, 9:00 AM Apr 10th, 11:45 AM

Comparison of Potency of SOCE Inhibition by Naturally Occurring Biphenolic Compounds

Upper Atrium/Hallway

Asian herbal medicine has used plants to treat many diseases for years. Two main compounds derived from Magnolia trees, honokiol and magnolol, have become accepted in their treatment of mental disorders such as anxiety and depression. These compounds have more recently been studied for their unique abilities to induce apoptosis and cytotoxicity in cancer cell lines. The purpose of this study was to determine and compare how honokiol, its sister compound, magnolol, and two other isomers, compound 24 and 27, affect intracellular calcium concentrations in cells expressing muscarinic receptors. Calcium concentrations were determined using a Fura-2 ratiometric imaging protocol. It was determined that all four compounds inhibit SOCE, an ER calcium-replenishing mechanism, known to play a crucial role in apoptosis and cell cycle regulation. Of this, compound 27 was found to be the most potent. It is recommended that the isomers of honokiol be specifically looked at for their anti-tumorigenic properties.