Title

Effects of Chlorothiazide on Muscarinic Receptors

Presenter Information

Brittany Brand

Department

Biological Sciences

Major

Biology

Research Advisor

Aronstam, Robert

Advisor's Department

Biological Sciences

Funding Source

cDNA Resource lab

Abstract

Chlorothiazide (6-chloro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide) is diuretic drug normally used within a hospital setting to manage excess fluid associated with congestive heart failure and as an antihypertensive drug. The goal of this experiment was to define the effects of Chlorothiazide on muscarinic receptors. We determined the effects of Chlorothiazide on calcium signaling by using ratiometric fluorescent dye on Chinese hamster ovary (CHO) cells, which have the human M3 muscarinic receptors, and imaging the amounts of intracellular calcium concentrations released. Based on the results of the experiment conducted, Chlorothiazide has no legitimate inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. Chlorothiazide most likely acts as a stimulant to the uptake of calcium.

Biography

Brittany is a senior biological sciences student at Missouri University of Science and Technology. Brittany has volunteered as a student researcher for eleven months in the cDNA research center and greatly enjoys the research opportunities introduced to her by volunteering in the lab.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hallway

Presentation Date

10 Apr 2012, 9:00 am - 11:45 am

Comments

Joint project with Katherine Bey

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Apr 10th, 9:00 AM Apr 10th, 11:45 AM

Effects of Chlorothiazide on Muscarinic Receptors

Upper Atrium/Hallway

Chlorothiazide (6-chloro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide) is diuretic drug normally used within a hospital setting to manage excess fluid associated with congestive heart failure and as an antihypertensive drug. The goal of this experiment was to define the effects of Chlorothiazide on muscarinic receptors. We determined the effects of Chlorothiazide on calcium signaling by using ratiometric fluorescent dye on Chinese hamster ovary (CHO) cells, which have the human M3 muscarinic receptors, and imaging the amounts of intracellular calcium concentrations released. Based on the results of the experiment conducted, Chlorothiazide has no legitimate inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. Chlorothiazide most likely acts as a stimulant to the uptake of calcium.