Title

Changes in Gene Expression of Muscarinic Acetylcholine Receptors Mediated by a Constitutively Active Phenotype

Presenter Information

Erica Shannon

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Aronstam, Robert

Advisor's Department

Biological Sciences

Funding Source

Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program; Biological Sciences Department; Missouri S&T cDNA Resource Center

Abstract

Muscarinic acetylcholine receptors are involved in multiple processes of the central and autonomic nervous system. They play an integral role in learning, memory, mood, attention, arousal, rest, and digestion. Acetylcholine, the native agonist for muscarinic receptors, initiates a signal transduction pathway that begins in the extracellular matrix and culminates with altered gene expression in the nucleus of the cell. These changes in gene expression can be measured using reporter plasmid comprised of specific response elements coupled to the gene coding for firefly luciferase. In these assays, the amount of light emitted directly correlates with the concentration of luciferase, a measure of response element activation. In the present study I demonstrate that each of the five muscarinic receptors subtypes with the constitutively active phenotype increase unstimulated gene expression and a potentiation of the induced reporter gene expression.

Biography

Erica is a junior in the Biological Sciences department at Missouri S&T. She is pursuing a BS in Biological Sciences and minors in Chemistry, Psychology of Leadership, and Organizational Psychology. Since her freshman year Erica has been actively involved in student organizations and held many leadership roles. Erica is currently the Webmaster of the National Residence Hall Honorary (NRHH) Shamrock Chapter and President of the international Genetically Engineered Machines (iGEM) Student Design Team. In addition to participating in student organizations, Erica is a student researcher in the biological sciences neurobiology lab and a student worker in the cDNA Resource Center. In her free time Erica likes to paint and read Michael Crichton novels.

Research Category

Sciences

Presentation Type

Oral Presentation

Document Type

Presentation

Award

Sciences oral presentation, First place
Best Bibliography Award Recipient, Sciences

Location

Turner Room

Presentation Date

06 Apr 2011, 9:00 am - 9:30 am

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Apr 6th, 9:00 AM Apr 6th, 9:30 AM

Changes in Gene Expression of Muscarinic Acetylcholine Receptors Mediated by a Constitutively Active Phenotype

Turner Room

Muscarinic acetylcholine receptors are involved in multiple processes of the central and autonomic nervous system. They play an integral role in learning, memory, mood, attention, arousal, rest, and digestion. Acetylcholine, the native agonist for muscarinic receptors, initiates a signal transduction pathway that begins in the extracellular matrix and culminates with altered gene expression in the nucleus of the cell. These changes in gene expression can be measured using reporter plasmid comprised of specific response elements coupled to the gene coding for firefly luciferase. In these assays, the amount of light emitted directly correlates with the concentration of luciferase, a measure of response element activation. In the present study I demonstrate that each of the five muscarinic receptors subtypes with the constitutively active phenotype increase unstimulated gene expression and a potentiation of the induced reporter gene expression.