Title

Isolation and implementation of the electron shuttling pathway from Geobacter into Escherichia coli

Presenter Information

Meghan Ray

Department

Chemical and Biochemical Engineering

Major

Chemical Engineering

Research Advisor

Westenberg, David J.

Advisor's Department

Biological Sciences

Funding Source

Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program; Missouri S&T Department of Biological Sciences; Missouri S&T Department of Chemical and Biological Engineering; Energy Research and Development Center; Materials Research Center

Abstract

The growing need for alternative fuel sources has sparked interest and research across many scientific and engineering disciplines. The fledgling field of microbial fuel cell development has previously relied on anaerobic metal reducing organisms such as Geobacter sulfurreduccens. This project sought to isolate the electron shuttling pathway in Geobacter and transform them into the more manageable aerobic Escherichia coli. The Missouri University of Science and Technology iGEM team isolated four outer membrane cytochrome (omc) genes from Geobacter, vital to the extracellular transportation of electrons. The four genes; B, E, S and T, were cloned into individual plasmids. The eventual goal is to combine all four genes into one plasmid to transform into E. coli to create an aerobic, electron transporting microbial system.

Biography

Meghan Ray is a junior in Biochemical Engineering from Havana, Illinois. She is a student leader in many organizations including W. T. Schrenk Society, Alpha Chi Sigma, and iGEM. She is also an active member of Kappa Delta Sorority.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Award

Sciences poster session, Second place

Location

Upper Atrium/Hallway

Presentation Date

07 Apr 2010, 9:00 am - 11:45 am

Comments

Joint Project with Nichole Hurd and Daniel Roush

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Apr 7th, 9:00 AM Apr 7th, 11:45 AM

Isolation and implementation of the electron shuttling pathway from Geobacter into Escherichia coli

Upper Atrium/Hallway

The growing need for alternative fuel sources has sparked interest and research across many scientific and engineering disciplines. The fledgling field of microbial fuel cell development has previously relied on anaerobic metal reducing organisms such as Geobacter sulfurreduccens. This project sought to isolate the electron shuttling pathway in Geobacter and transform them into the more manageable aerobic Escherichia coli. The Missouri University of Science and Technology iGEM team isolated four outer membrane cytochrome (omc) genes from Geobacter, vital to the extracellular transportation of electrons. The four genes; B, E, S and T, were cloned into individual plasmids. The eventual goal is to combine all four genes into one plasmid to transform into E. coli to create an aerobic, electron transporting microbial system.