Title

Regulation of Actomyosin Ring Formation in Budding Yeast

Presenter Information

Joseph Karas

Department

Biological Sciences

Major

Biological Sciences and Chemistry

Research Advisor

Shannon, Katie

Advisor's Department

Biological Sciences

Funding Source

Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program

Abstract

Our research lab is interested in cytokinesis, the division of a cell into two daughter cells. Cytokinesis failure results in polyploidy, which may contribute to tumorigenesis or cause cell death.

My research project is focused on IQG1. IQG1 is a protein required for cytokinesis in budding yeast. IQG1 is necessary to recruit actin to the actomyosin ring at the division site during M phase. I have tested the hypothesis that overexpression of IQG1 can promote premature actin ring formation. Using a strain with IQG1 under control of the inducible GAL1 promoter, cells were synchronized using [missing character] factor, fixed at numerous time points as cells progress through mitosis, and stained for actin to determine the timing of actin ring formation. My data does not show premature actin ring formation as a result of IQG1 overexpression. An alternative hypothesis is that actin ring formation is regulated by Mlc1. Mlc1 is a myosin light chain that binds IQG1 and is required for IQG1 localization. I am currently creating a strain overexpressing Mlc1 to test this hypothesis.

Biography

Joseph Karas is a senior Biological Sciences major from Chicago, IL. He has worked for a year in Dr. Shannon’s cytokinesis lab. Joe has also worked during the summer as an industrial chemist. Joe was on the Missouri S&T Cross country team and worked for the school newspaper.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hallway

Presentation Date

08 Apr 2009, 9:00 am - 11:45 am

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Apr 8th, 9:00 AM Apr 8th, 11:45 AM

Regulation of Actomyosin Ring Formation in Budding Yeast

Upper Atrium/Hallway

Our research lab is interested in cytokinesis, the division of a cell into two daughter cells. Cytokinesis failure results in polyploidy, which may contribute to tumorigenesis or cause cell death.

My research project is focused on IQG1. IQG1 is a protein required for cytokinesis in budding yeast. IQG1 is necessary to recruit actin to the actomyosin ring at the division site during M phase. I have tested the hypothesis that overexpression of IQG1 can promote premature actin ring formation. Using a strain with IQG1 under control of the inducible GAL1 promoter, cells were synchronized using [missing character] factor, fixed at numerous time points as cells progress through mitosis, and stained for actin to determine the timing of actin ring formation. My data does not show premature actin ring formation as a result of IQG1 overexpression. An alternative hypothesis is that actin ring formation is regulated by Mlc1. Mlc1 is a myosin light chain that binds IQG1 and is required for IQG1 localization. I am currently creating a strain overexpressing Mlc1 to test this hypothesis.