Title

The Growth of MC3T3-E1 Pre-Osteoblasts on Borate Containing Glasses

Presenter Information

Agatja Agatha Dwilewicz

Department

Biological Sciences

Major

Biological Science

Research Advisor

Brown, Roger F.
Rahaman, M. N., 1950-

Advisor's Department

Biological Sciences

Second Advisor's Department

Materials Science and Engineering

Funding Source

Musculoskeletal Transplant Foundation (Rahaman, PI)

Abstract

Borate-based glasses in the B2O3-SiO2-Na2O-CaO-P2O5 system were recently developed at this institution for possible use as scaffolds in bone tissue engineering. In this study, three of the borate-based glasses, compositions designated 1B, 2B, and 3B, with B2O3 levels of 15, 31, and 46 mole percent, respectively, were tested for their effects on the growth of MC3T3-E1 mouse pre-osteoblastic cells. Silicate type 45S5 glass was used as a control for comparison. Tests performed included: contact assays of cell growth at the glass interface, quantitative fluorescence assay of cell proliferation in the presence of the test glasses, chemical analysis of borate released into the culture medium, and scanning electron microscope imaging. The results obtained suggest that 1B and 2B glasses permit a reasonable level of MC3T3-E1 cell growth and appear promising for possible use in bone tissue engineering.

Biography

Agatha Dwilewicz is a sophomore undergraduate student at the University of Missouri-Rolla, majoring in Biological Sciences.

Research Category

Natural Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Award

Natural Sciences poster session, Second place

Presentation Date

12 Apr 2006, 1:00 pm

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Apr 12th, 1:00 PM

The Growth of MC3T3-E1 Pre-Osteoblasts on Borate Containing Glasses

Borate-based glasses in the B2O3-SiO2-Na2O-CaO-P2O5 system were recently developed at this institution for possible use as scaffolds in bone tissue engineering. In this study, three of the borate-based glasses, compositions designated 1B, 2B, and 3B, with B2O3 levels of 15, 31, and 46 mole percent, respectively, were tested for their effects on the growth of MC3T3-E1 mouse pre-osteoblastic cells. Silicate type 45S5 glass was used as a control for comparison. Tests performed included: contact assays of cell growth at the glass interface, quantitative fluorescence assay of cell proliferation in the presence of the test glasses, chemical analysis of borate released into the culture medium, and scanning electron microscope imaging. The results obtained suggest that 1B and 2B glasses permit a reasonable level of MC3T3-E1 cell growth and appear promising for possible use in bone tissue engineering.