Location

Innovation Lab, Room 212

Start Date

4-2-2025 2:00 PM

End Date

4-2-2025 2:30 PM

Presentation Date

2 April 2025; 2:00pm - 2:30pm

Biography

Arnold biography:

Carson Arnold is a third-year biological sciences major in the OURE program working with Biological Sciences professor, Dave Duvernell as his advisor. Carson is from the small town in Southeast Missouri called Doniphan. He graduated from Doniphan High School and began studying biological sciences. Carson has a passion for working with and studying animals with a particular interest in fish. As such, he joined Dr. Duvernell's research on fish hybridization, beginning an OURE project on Missouri Minnows, which involved traveling much of the state collecting specimens to build a detailed phylogeny

Koju biography:

This biography is for Shila Koju. I am a junior undergraduate international student from Nepal majoring in Biological Sciences, emphasis in Medical Lab Science and minor in Chemistry. I am a first-generation college student from Nepal. Coming here was a big leap of faith for me, but it has turned worthwhile; all thanks to the S&T community and all the amazing opportunities it provides me with- one of it being undergraduate research experience. I can’t wait to explore and excel everything this campus has to offer in my remainder of time here.

Meeting Name

2025 - Miners Solving for Tomorrow Research Conference

Department(s)

Biological Sciences

Comments

Advisor: David Duvernell

Abstract:

Hybridization is common among minnow species with historical precedent for mitochondrial introgression events throughout the Luxilus genus. Within the Ozarks, hybrid introgression has led to L. chrysocephalus populations that exhibit the mitogenomes of a congener, L. cornutus. Preliminary surveys of another shiner species, L. zonatus, suggested another hybrid mitochondrial genome replacement with L. chrysocephalus. The objective of our study was to sample L. zonatus and L. chrysocephalus throughout the Ozarks to document the geographic extent of hybrid introgression. We sequenced the ND2 gene and constructed a maximum likelihood mitochondrial phylogeny. Our work revealed that only L. zonatus from the Black River drainage in the southern Ozarks formed a monophyletic group with sister species L. pilsbryi and L. cardinalis. Populations of L. zonatus from the St. Francis, Meramec, Gasconade and Osage Rivers all exhibit the mitogenomes of L. chrysocephalus. This is interesting because L. chrysocephalus in those drainages possess L. cornutus mitogenomes. We hypothesize that historical hybrid introgression between L. chrysocephalus and L. zonatus preceded hybrid introgression between L. cornutus and L. chrysocephalus to account for these findings. Our results have practical implications because the use of mitochondrial genomes for environmental DNA metabarcoding has become widespread and introgressive hybridization events such as these have the potential to cause misidentification of species. Supported by Missouri S&T OURE program.

Document Type

Presentation

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2025 The Authors, all rights reserved

Arnold_slides.pdf (1417 kB)

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Apr 2nd, 2:00 PM Apr 2nd, 2:30 PM

Hybrid Introgression has resulted in Bleeding Shiners exhibiting Striped Shiner mitogenomes in certain Missouri Ozark streams

Innovation Lab, Room 212