In Vitro Stimulation of Vascular Endothelial Growth Factor by Borate-Based Glass Fibers under Dynamic Flow Conditions


Bioactive borate glass has been recognized to have both hard and soft tissue repair and regeneration capabilities through stimulating both osteogenesis and angiogenesis. However, the underlying biochemical and cellular mechanisms remain unclear. In this study, dynamic flow culturing modules were designed to simulate the micro-environment near the vascular depletion and hyperplasia area in wound-healing regions, thus to better investigate the mechanisms underlying the biocompatibility and functionality of borate-based glass materials. Glass fibers were dosed either upstream or in contact with the pre-seeded cells in the dynamic flow module. Two types of borate glasses, doped with (1605) or without (13-93B3) CuO and ZnO, were studied along with the silicate-based glass, 45S5. Substantial fiber dissolution in cell culture medium was observed, leading to the release of ions (boron, sodium and potassium) and the deposition of a calcium phosphate phase. Different levels of vascular endothelial growth factor secretion were observed from cells exposed to these three glass fibers, and the copper/zinc containing borate 1605 fibers exhibited the most positive influence. These results indicate that dynamic studies of in vitro bioactivity provide useful information to understand the in vivo response to bioactive borate glasses.


Materials Science and Engineering

Second Department


Third Department

Electrical and Computer Engineering

Keywords and Phrases

Bioactive Borate Glass Nano-/Micro-Fibers; Copper/Zinc (Cu/Zn) Doped Glass Fibers; Dynamic Flow Culturing Module; Vascular Endothelial Growth Factor (VEGF); Wound Healing

International Standard Serial Number (ISSN)


Document Type

Article - Journal

Document Version


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© 2017 Elsevier B.V., All rights reserved.

Publication Date

01 Apr 2017