Radiant Star Nanoparticle Prodrugs for the Treatment of Intracellular Alveolar Infections
Radiant star nanoparticle (RSN) prodrugs were synthesized in a two-step process by first homopolymerizing RAFT transmers followed by copolymerization from the hyperbranched polymer core. Two trithiocarbonate-based transmers were synthesized containing either alkyl ester or acetal groups linking the polymerizable methacrylate group to the chain transfer agent (CTA). RAFT polymerization from the homopolymerized transmer cores yielded RSNs with linear polymer chains connected to hyperbranched cores. Hydrolysis studies conducted over a period of 30 days at 37 °C in acetate buffer showed that RSNs prepared from alkyl ester linked cores remained stable while acetal linked cores exhibited a progressive degradation into linear polymers over the same period. Macrophage targeting RSN prodrugs containing the antibiotic ciprofloxacin and receptor-targeting mannose residues were synthesized directly via RAFT polymerization of the prodrug and mannose monomers. Hydrolysis studies conducted in human serum showed that the RSNs released the covalently linked ciprofloxacin significantly faster than diblock copolymer micelles but moderately slower than soluble copolymers with comparable compositions. Flow cytometry showed substantially higher macrophage binding by the mannose-targeted RSNs while in vivo biocompatibility experiments showed no differences relative to phosphate buffer treated negative controls.
D. Das and S. Srinivasan and F. D. Brown and F. Y. Su and A. L. Burrell and J. M. Kollman and A. Postma and D. M. Ratner and P. S. Stayton and A. J. Convertine, "Radiant Star Nanoparticle Prodrugs for the Treatment of Intracellular Alveolar Infections," Polymer Chemistry, vol. 9, no. 16, pp. 2134 - 2146, Royal Society of Chemistry, Apr 2018.
The definitive version is available at https://doi.org/10.1039/c8py00202a
Materials Science and Engineering
Keywords and Phrases
Antibiotics; Biocompatibility; Dendrimers; Esters; Hydrolysis; Living polymerization; Macrophages; Nanoparticles; Stars, Chain transfer agents; Diblock copolymer micelles; Hyperbranched polymers; Linear polymer chains; Macrophage targeting; Methacrylate groups; RAft polymerization; Receptor targeting, Synthesis (chemical)
International Standard Serial Number (ISSN)
Article - Journal
© 2018 Royal Society of Chemistry, All rights reserved.
01 Apr 2018
This work was funded by the Defense Threat Reduction Agency (Grant #HDTRA1-13-1-0047).