Masters Theses

Keywords and Phrases

Restricted Diffusion; Porous Adsorbents; Relative Permeability; Biospecific Adsorption


"A restricted pore diffusion model is presented and employed as the principle theoretical tool to investigate salient characteristics of the permeability behavior of biologically active macromolecules in porous affinity chromatography media, when these large adsorbate molecules are adsorbed onto available vacant ligands immobilized therein. The contributions of steric hindrance at the entrance to the pores and frictional resistance within the pores to the overall restriction to pore diffusion, accompanied by the effects of pore size distribution, pore connectivity of the adsorbent, molecular dimension of adsorbate and ligand, and the fractional saturation of adsorption sites (ligands), are considered. Porous affinity matrices having dilute and high ligand concentrations are examined, and the permeability of the adsorbate in porous networks of connectivity nT is studied by means of effective medium approximation (EMA) numerical solutions. The detailed trends of the permeability of the adsorbate encountered in affinity adsorption systems of Porous Silica and Superose™ 6 with dilute and high ligand concentrations, are described and presented in numerous Tables. Results of this work may be of importance in studies involving the modeling, prediction of the dynamic behavior, design, and control of affinity chromatography (biospecific adsorption) adsorbents"--Abstract, page iii.


Liapis, Athanasios I.

Committee Member(s)

Findley, Marshall E., 1927-1991
Siehr, Donald J.
Rigler, A. K.


Chemical and Biochemical Engineering

Degree Name

M.S. in Chemical Engineering


North Atlantic Treaty Organization. Scientific Affairs Division
Weldon Spring Fund


This work was supported in part by a Grant from the Weldon Spring Fund and the NATO Scientific Affairs Division under Grant No. 0770/88.


University of Missouri--Rolla

Publication Date

Summer 1989


xii, 109 pages

Note about bibliography

Includes bibliographical references (pages 36-44).


© 1989 Nikolaos Panagiotis Kolliopoulos, All rights reserved.

Document Type

Thesis - Restricted Access

File Type




Subject Headings

Diffusion -- Mathematical models
Molecules -- Analysis
Chromatography, Affinity

Thesis Number

T 5900

Print OCLC #


Electronic OCLC #


Link to Catalog Record

Electronic access to the full-text of this document is restricted to Missouri S&T users. Otherwise, request this publication directly from Missouri S&T Library or contact your local library.

Share My Thesis If you are the author of this work and would like to grant permission to make it openly accessible to all, please click the button above.