"Seventy-two biochemical reactors were set up, and operated using a mixture of chip-bark, horse manure, and gravel as the biochemical treatment substrate. The simulated mine water containing sulfate (1000 mg/l) was pumped into each reactor at a flow rate of 0.5 ml/minute (approximate), giving an empty bed contact time of 8 days. The main idea is that the microorganisms present in horse manure would convert the cellulose in chip-bark into volatile fatty acids. The produced volatile fatty acid would enhance the metabolism of sulfate-reducing bacteria (SRB) initially present in horse manure, which would degrade (and eventually remove) the sulfate from mine-impacted water. At the end of every month, the amount of cellulose remaining in chip-bark samples were calculated using two different methods: NMR, and chemical extraction (acid-base-acid).
An objective of the experiment is analyzing the correlation between cellulose, and sulfate degradation rate. Separately, the ozonation method was evaluated as a potential surrogate for much slower biological degradation. It may be possible to predict the higher degradation rate of cellulose, and hence, degrade (or remove) the sulfate from mine-impacted water using the proposed biochemical process. The percentage of cellulose in fresh chip bark was 52%. There was degradation of 4.5 % cellulose in five months. First order kinetic equation was used to predict the time for exhaustion of cellulose. The time predicted by the designed model is 5 years to react with the maximum degradation (observed by ozone treatment) of chip bark"--Abstract, page iii.
Fitch, Mark W.
Burken, Joel G. (Joel Gerard)
Civil, Architectural and Environmental Engineering
M.S. in Environmental Engineering
Missouri University of Science and Technology
xii, 100 pages
© 2018 Sweta Ojha, All rights reserved.
Thesis - Open Access
Electronic OCLC #
Ojha, Sweta, "Cellulose and sulfate degradation in a biochemical reactor during treatment of mine drainage" (2018). Masters Theses. 7774.