Keywords and Phrases
N-acetylcysteine amide (NACA)
"Doxorubicin is a representative of the class of quinoid anthracycline antitumor drugs that are most widely used in cancer chemotherapy. Unfortunately, long-term treatment by doxorubicin is limited by cardiotoxicity due to generation of reactive oxygen species (ROS). This study was conducted to determine the effects of a new thiol antioxidant, N-acetylcysteine amide (NACA), on doxorubicin-induced cardiotoxicity. Our results suggest that doxorubicin (5/[mu]M) induces cardiotoxicity by increasing generation of ROS, decreasing levels of glutathione (GSH) and MTS, an important cell viability indicator, increasing malondialdehyde (MDA) levels and caspase-3 activity, decreasing activities of antioxidant enzymes, such as catalase, glutathione peroxidase and glutathione reductase and inducing apoptosis. NACA protected H9c2 cells from doxorubicin-induced cardiotoxicity, as evaluated by MTS assay. It also significantly reduced the generation of ROS and decreased MDA in doxorubicin-treated cells. In addition, NACA increased the GSH level, as well as the GSH/GSSG ratio, and the increased caspase-3 activity was significantly decreased by NACA. The activities of antioxidant enzymes were also reduced by NACA"--Abstract, page iii.
M.S. in Chemistry
University of Missouri--Rolla
ix, 61 pages
© 2006 Rong Shi, All rights reserved.
Thesis - Restricted Access
Antineoplastic agents -- Toxicology
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Link to Catalog Record
Electronic access to the full-text of this document is restricted to Missouri S&T users. Otherwise, request this publication directly from Missouri S&T Library or contact your local library.http://merlin.lib.umsystem.edu/record=b5999603~S5
Shi, Rong, "Effects of a newly synthesized thiol antioxidant on doxorubicin-induced oxidative stress" (2006). Masters Theses. 5904.
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