"The product formed from tryptamine by Trichloloma nudum was identified as indoleacetic acid (IAA) and from tryptophan by Cantharellus cibrarius as indolelactic acid (ILA). The products were extracted from the spent media with organic solvents. Purification and identification of the crystalline transformation products were accomplished by means of paper chromatography, spectrophotometry and thin layer chromatography.
The conversion of tryptamine and tryptophan was also studied in cultures of Lencopaxillus paradoxus, Pluteus granularis and Pleuatus ostreatus. Only Pluteus granularis was found to transform tryptamine into IAA. Lencopaxillus paradoxus transformed tryptamine into oxindoleacetic acid (OIAA).
Paper chromatography proved to be useful for the qualitative identification of indoleacetic acid, indolelactic acid, oxindole-acetic acid and anthranilic acid. Spectrophotometry was employed for the quantitative estimation of the substances formed"--Abstract, page ii.
Siehr, Donald J.
Conrad, Frank H., 1902-1983
Webb, William H.
Lorey, G. Edwin
M.S. in Chemistry
University of Missouri at Rolla
vi, 41 pages
© 1965 Hsiao Lung Cheng, All rights reserved.
Thesis - Open Access
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Cheng, Hsaio-lung, "Transformations of tryptamine and tryptophan by basidiomycetes" (1965). Masters Theses. 5690.