Masters Theses

Abstract

"This investigation consisted of two parts focused on bioactive glasses and scaffolds for in vitro growth of bone cells for tissue engineering and bone repair. The first part involved evaluation of the ability of two new types of porous 13-93 bioactive glass scaffolds to support the growth and differentiation of mouse MLO-AS cells, an established osteogenic cell line used as a model of developing bone tissue. The two scaffold types tested included 13-93 glass fiber and trabecular-like scaffolds seeded with MLO-AS cells and cultured for intervals of 2 to 12 days. Collectively, in vitro results indicate that the 13-93 glass fiber and trabecular scaffolds promote the attachment, growth, and differentiation of MLO-AS osteogenic cells and could potentially be used for bone tissue engineering applications. The second part of the investigation involved in vitro testing of modified compositions of 45S5 and 13-93 glasses with MLO-AS osteogenic cells. The modified glasses, which were designed for use as cell support scaffolds, contained added boron (to form the glasses 13-93 Bl and 13-93 B3) or various trace elements such as copper and zinc (forming the glasses 13-93 TE, 45S5 TE, and 45S5 TE (900C)). Quantitative and qualitative tests revealed no inhibition of growth of MLO-AS cells incubated with 13-93, 13-93 TE, and 45S5 TE extracts. Tests showed moderate inhibition of growth by 13-93 Bland 45S5 extracts and >75% inhibition of growth by 13-93 B3 and 45S5 TE (900C) extracts. However, dynamic incubation of cell-seeded 13-93 B3 scaffolds reduced cytotoxic effects, suggesting that cell growth on this glass may be possible under dynamic conditions including the environment provided by the in vivo microvasculature"--Abstract, page iv.

Advisor(s)

Brown, Roger F.

Committee Member(s)

Maglia, Anne M.
Day, D. E.

Department(s)

Biological Sciences

Degree Name

M.S. in Applied and Environmental Biology

Sponsor(s)

Missouri University of Science and Technology. Center for Bone and Tissue Repair and Regeneration

Publisher

Missouri University of Science and Technology

Publication Date

Summer 2009

Journal article titles appearing in thesis/dissertation

  • In Vitro Performance Of 13-93 Bioactive Glass Fiber And Trabecular Scaffolds With MLO-A5 Osteogenic Cells
  • Cytotoxicity Assessment Of Modified Bioactive Glasses And Fiber Scaffolds With MLO-A5 Osteogenic Cells In Vitro

Pagination

x, 72 pages

Note about bibliography

Includes bibliographical references.

Rights

© 2009 Vernon Christopher Modglin, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Subject Headings

Tissue scaffolds
Glass in medicine
Biomedical materials -- Testing
Bioactive compounds
Bone regeneration
Bones -- Growth
Tissue engineering

Thesis Number

T 10230

Print OCLC #

863465304

Electronic OCLC #

905981017

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