Immunohistochemistry and RNA in Situ Hybridization in Mouse Brain Development
Abstract
During development, the mouse brain is progressively divided into functionally distinct compartments. Numerous neuronal and glial cell types are subsequently generated in response to various inductive signals. Each cell expresses a unique combination of genes encoding proteins from transcription factors to neurotransmitters that define its role in brain function. To understand these important and highly sophisticated processes, it is critical to accurately locate the various proteins and cells that produce them. In this chapter, we introduce the techniques of Immunohistochemistry, which detects the localization of specific proteins, and RNA in situ hybridization, which enables the visualization of specific mRNAs.
Recommended Citation
J. Liu and A. Liu, "Immunohistochemistry and RNA in Situ Hybridization in Mouse Brain Development," Brain Development: Methods and Protocols, vol. 2047, pp. 475 - 489, Humana Press Inc., Jan 2020.
The definitive version is available at https://doi.org/10.1007/978-1-4939-9732-9_27
Department(s)
Engineering Management and Systems Engineering
Second Department
Biological Sciences
Research Center/Lab(s)
Center for High Performance Computing Research
Second Research Center/Lab
Intelligent Systems Center
Keywords and Phrases
Antibody; Cryosection; Digoxigenin; Fluorescent; Immunohistochemistry; RNA in situ hybridization
International Standard Serial Number (ISSN)
1064-3745
Document Type
Book - Chapter
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2020 Springer Science+Business Media, LLC, All rights reserved.
Publication Date
01 Jan 2020
PubMed ID
31552672
Comments
Part of the Methods in Molecular Biology book series (MIMB, volume 2047)