Title

Immunohistochemistry and RNA in Situ Hybridization in Mouse Brain Development

Abstract

During development, the mouse brain is progressively divided into functionally distinct compartments. Numerous neuronal and glial cell types are subsequently generated in response to various inductive signals. Each cell expresses a unique combination of genes encoding proteins from transcription factors to neurotransmitters that define its role in brain function. To understand these important and highly sophisticated processes, it is critical to accurately locate the various proteins and cells that produce them. In this chapter, we introduce the techniques of Immunohistochemistry, which detects the localization of specific proteins, and RNA in situ hybridization, which enables the visualization of specific mRNAs.

Department(s)

Engineering Management and Systems Engineering

Second Department

Biological Sciences

Comments

Part of the Methods in Molecular Biology book series (MIMB, volume 2047)

Keywords and Phrases

Antibody; Cryosection; Digoxigenin; Fluorescent; Immunohistochemistry; RNA in situ hybridization

International Standard Serial Number (ISSN)

1064-3745

Document Type

Book - Chapter

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2020 Springer Science+Business Media, LLC, All rights reserved.

Publication Date

01 Jan 2020

PubMed ID

31552672

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