Hedgehog signaling is crucial for vertebrate development and physiology. Gli2, the primary effector of Hedgehog signaling, localizes to the tip of the primary cilium, but the importance of its ciliary localization remains unclear. We address the roles of Gli2 ciliary localization by replacing endogenous Gli2 with Gli2ΔCLR, a Gli2 variant not localizing to the cilium. The resulting Gli2ΔCLRKI and Gli2ΔCLRKI;Gli3 double mutants resemble Gli2-null and Gli2;Gli3 double mutants, respectively, suggesting the lack of Gli2ΔCLR activation in development. Significantly, Gli2ΔCLR cannot be activated either by pharmacochemical activation of Smo in vitro or by loss of Ptch1 in vivo. Finally,Gli2ΔCLR exhibits strong transcriptional activator activity in the absence of Sufu, suggesting that the lack of its activation in vivo results from a specific failure in relieving the inhibitory function of Sufu. Our results provide strong evidence that the ciliary localization of Gli2 is crucial for cilium-dependent activation of Hedgehog signaling.


Engineering Management and Systems Engineering

Second Department

Biological Sciences

Keywords and Phrases

Cilium; Hedgehog signaling; Mouse; Spinal cord; Sufu

International Standard Serial Number (ISSN)

0950-1991; 1477-9129

Document Type

Article - Journal

Document Version

Final Version

File Type





© 2015 Company of Biologists Ltd, All rights reserved.

Publication Date

01 Mar 2015

PubMed ID


Included in

Biology Commons