Biochemical sensors for continuous monitoring require dependable periodic self diagnosis with acceptable simplicity to check its functionality during operation. An in-situ self-diagnostic technique for a dissolved oxygen microsensor is proposed in an effort to devise an intelligent microsensor system with an integrated electrochemical actuation electrode. With a built-in platinum microelectrode that surrounds the microsensor, two kinds of microenvironments, called the oxygen-saturated or oxygen-depleted phases, can be created by water electrolysis, depending on the polarity. The functionality of the microsensor can be checked during these microenvironment phases. The polarographic oxygen microsensor is fabricated on a flexible polyimide substrate (Kapton) and the feasibility of the proposed concept is demonstrated in a physiological solution. The sensor responds properly during the oxygen-generating and oxygen-depleting phases. The use of these microenvironments for in-situ self-calibration is discussed to achieve functional integration, as well as structural integration, of the microsensor system.


Electrical and Computer Engineering

Second Department

Chemical and Biochemical Engineering

Keywords and Phrases

Electrolysis; Biochemical Sensors; Biosensors; Dissolved Oxygen Microsensor; Electrochemical Actuator; Electrochemical Electrodes; Electrochemical Sensors; Electrolysis; In-Situ Self-Calibration; Intelligent Microsensor; Intelligent Sensors; Microenvironment Manipulation; Microsensors; Oxygen; Oxygen-Depleted Phase; Oxygen-Saturated Phase; Periodic Self Diagnosis; Polarographic Oxygen Microsensor; Polyimide; Polyimide Substrate; Self Calibration; Self Diagnosis; Water Electrolysis

International Standard Serial Number (ISSN)


Document Type

Article - Journal

Document Version

Final Version

File Type





© 2004 Institute of Electrical and Electronics Engineers (IEEE), All rights reserved.

Publication Date

01 Jan 2004