Unpredictable baseline drift and sensitivity degradation during continuous use are two of the most significant problems of biosensors including the amperometric glucose and lactate sensors. Therefore, the capability of on-demand in situ calibration/diagnosis of biochemical sensors is indispensable for reliable long-term monitoring with minimum attendance. Another limitation of oxidase enzyme-based biosensors is the dependence of enzyme activity on the background oxygen concentration in sample solution. In order to address these issues, the electrolytic generation of oxygen and hydrogen bubbles were utilized 1) to overcome the background oxygen dependence of glucose and lactate sensors and 2) to demonstrate the feasibility of in situ self-calibration of the proposed glucose and lactate sensors. Experimental data assure that the proposed techniques effectively establish the zero calibration value and significantly improve the measurement sensitivity and dynamic range in both glucose and lactate sensors.


Electrical and Computer Engineering

Second Department

Biological Sciences


National Science Foundation (U.S.)
University of Missouri--Rolla. Intelligent Systems Center


This work was supported in part by the National Science Foundation (NSF) under ECS-0400913 and NSF ECS-0427360 and by the Intelligent Systems Center (ISC) at UMR.

Keywords and Phrases

Actuator; Amperometric Sensors; Baseline Drift; Biosensors; Calibration; Electrochemical Actuation System; Electrolytic Generation; Enzyme Activity; Enzymes; Glucose Oxidase (GOD); Lactate Oxidase (LOD); Oxidase Enzyme; Sensitivity; Sensitivity Degradation; Sensors Diagnosis; Solid-State Biosensor; Sugar; Thin-Film Amperometric Sensor; Water Electrolysis

International Standard Serial Number (ISSN)

0018-9456; 1557-9662

Document Type

Article - Journal

Document Version

Final Version

File Type





© 2006 Institute of Electrical and Electronics Engineers (IEEE), All rights reserved.

Publication Date

01 Aug 2006