Doctoral Dissertations

Keywords and Phrases

Bioactive glass; Cytotoxicity; Fiber-optic micro-sensor; High-resolution pH probe; Manufactured nano-/micro-materials; Single cell heterogeneity


"Manufactured nano-/micro-materials (MNMs) have been widely used and their interactions with niche biological environment are highly concerned for both of their biohazardous and bioactive effects, whereas no available comprehensive evaluations or regulations have been provided yet. This dissertation thus focuses on three major aspects: 1) fundamental toxicity understandings of a typical MNMs (zinc oxide nanoparticles), 2) bioactivity evaluations of representative bioactive MNMs, and 3) development of novel micro-probes for high spatial resolution monitoring. Firstly, the NP's concentration, irradiation, hydrodynamic size, and the localized pH, ionic strength, NP zeta-potential as well as dissolved oxygen levels were found correlated with the production of hydroxyl radicals (•OH). Thus a novel physicochemical mechanism was hypothesized on •OH generation from ZnO NPs to cast light on cytotoxic mechanisms of MNMs. Secondly, silicate-/borate-based nano-/micro-sized glass fibers showed good rehabilitation capability and the underlying mechanisms were revealed as that quicker ion releasing and glass conversion (into hydroxyapatite (HA)) are the key to promote cell proliferation and migration, thus the wound-healing effect. Thirdly, several types of fiber-optic-based probes were developed to better cope with high spatial resolution, niche biological environmental detection. Up to date the best probe is able to acquire a pH resolution of up to ~0.02 pH unit within biologically relevant pH range of 6.17 - 8.11 with fast sensing time of ~5 seconds. Real-time monitoring of single live human cells were also demonstrated and validated in cytotoxic studies to detect early-onset of cell deterioration on different stages, indicating its powerful potent on studies that focusing on MNMs and single cells"--Abstract, page iv.


Ma, Yinfa

Committee Member(s)

Xiao, Hai, Dr.
Shi, Honglan
Winiarz, Jeffrey G.
Nam, Paul Ki-souk



Degree Name

Ph. D. in Chemistry


This study was funded by Environmental Research Center and the Center for Biomedical Science and Engineering at Missouri University of Science and Technology.

This project was supported by internal funding from the Center for Single Nanoparticle, Single Cell, and Single Molecule Monitoring (CS³M), and Department of Chemistry at Missouri University of Science and Technology.

This project was supported by the Center for Biomedical Science and Engineering (CBSE) and Center for Single Nanoparticle, Single cell, and Single Molecule Monitoring (CS3M), at Missouri University of Science and Technology.

TThis research was financially supported by National Institutes of Health grant (Award No. 1R21GM104696-01).


Missouri University of Science and Technology

Publication Date

Summer 2016

Journal article titles appearing in thesis/dissertation

  • Irradiation-enhanced cytotoxicity of zinc oxide nanoparticles
  • Physicochemical insights of irradiation-enhanced hydroxyl radical generation from ZnO nanoparticles
  • In vitro study of improved wound-healing effect of bioactive borate-based glass nano-/micro-fibers
  • Reflection-mode micro-spherical fiber-optic probes for in vitro real-time and single-cell level pH sensing
  • Fiber-optic-based micro-probe using hexagonal 1-in-6 fiber configuration for intracellular single-cell pH measurement
  • High spatiotemporal in situ pH monitoring in a single live cell using a micro-fiber-optic probe


xvi, 211 pages

Note about bibliography

Includes bibliographic references.


© 2016 Qingbo Yang, All rights reserved.

Document Type

Dissertation - Open Access

File Type




Thesis Number

T 11730

Electronic OCLC #