Keywords and Phrases
Bioactive glass; Cytotoxicity; Fiber-optic micro-sensor; High-resolution pH probe; Manufactured nano-/micro-materials; Single cell heterogeneity
"Manufactured nano-/micro-materials (MNMs) have been widely used and their interactions with niche biological environment are highly concerned for both of their biohazardous and bioactive effects, whereas no available comprehensive evaluations or regulations have been provided yet. This dissertation thus focuses on three major aspects: 1) fundamental toxicity understandings of a typical MNMs (zinc oxide nanoparticles), 2) bioactivity evaluations of representative bioactive MNMs, and 3) development of novel micro-probes for high spatial resolution monitoring. Firstly, the NP's concentration, irradiation, hydrodynamic size, and the localized pH, ionic strength, NP zeta-potential as well as dissolved oxygen levels were found correlated with the production of hydroxyl radicals (•OH). Thus a novel physicochemical mechanism was hypothesized on •OH generation from ZnO NPs to cast light on cytotoxic mechanisms of MNMs. Secondly, silicate-/borate-based nano-/micro-sized glass fibers showed good rehabilitation capability and the underlying mechanisms were revealed as that quicker ion releasing and glass conversion (into hydroxyapatite (HA)) are the key to promote cell proliferation and migration, thus the wound-healing effect. Thirdly, several types of fiber-optic-based probes were developed to better cope with high spatial resolution, niche biological environmental detection. Up to date the best probe is able to acquire a pH resolution of up to ~0.02 pH unit within biologically relevant pH range of 6.17 - 8.11 with fast sensing time of ~5 seconds. Real-time monitoring of single live human cells were also demonstrated and validated in cytotoxic studies to detect early-onset of cell deterioration on different stages, indicating its powerful potent on studies that focusing on MNMs and single cells"--Abstract, page iv.
Xiao, Hai, Dr.
Winiarz, Jeffrey G.
Nam, Paul Ki-souk
Ph. D. in Chemistry
Missouri University of Science and Technology
Journal article titles appearing in thesis/dissertation
- Irradiation-enhanced cytotoxicity of zinc oxide nanoparticles
- Physicochemical insights of irradiation-enhanced hydroxyl radical generation from ZnO nanoparticles
- In vitro study of improved wound-healing effect of bioactive borate-based glass nano-/micro-fibers
- Reflection-mode micro-spherical fiber-optic probes for in vitro real-time and single-cell level pH sensing
- Fiber-optic-based micro-probe using hexagonal 1-in-6 fiber configuration for intracellular single-cell pH measurement
- High spatiotemporal in situ pH monitoring in a single live cell using a micro-fiber-optic probe
xvi, 211 pages
© 2016 Qingbo Yang, All rights reserved.
Dissertation - Open Access
Electronic OCLC #
Yang, Qingbo, "Bioactivity/cytotoxicity of micro-/nano-materials and novel development of fiber-optic probes for single cell monitoring" (2016). Doctoral Dissertations. 2902.