The modelling and analysis of the dynamic performance of perfusion chromatography in fixed bed and periodic countercurrent column operations and parameter estimation and model discrimination using experimental data
"Comparisons of dynamic simulation results for single and multi-component adsorption in columns operated in fixed bed and periodic countercurrent mode show that the periodic countercurrent operating mode nearly always gives superior performance. Furthermore, the performance of columns operated in the periodic countercurrent mode can be independent of the values of parameters that determine the mass transfer resistances of the system.
Simulation results over a range of values of several adsorbent particle structural and adsorption mechanism parameters show that intraparticle fluid flow in perfusive particles can improve adsorption system performance if it is of sufficient magnitude. However, performance with purely diffusive particles can be better than with perfusive particles, if purely diffusive particles can be made with higher values of CT, the maximum equilibrium concentration of adsorbate in the adsorbed phase of the adsorbent particle, than otherwise comparable perfusive particles.
A proposed method for parameter estimation in the Laplace transform domain is able to estimate intraparticle mass transfer parameters in linear adsorption models from simulated experimental data. The dynamic nonlinear model for purely diffusive particles having a monodisperse porous structure describes most appropriately experimental breakthrough data for the adsorption of bovine serum albumin onto porous anion-exchange particles packed in a column"--Abstract, page iv.
Liapis, Athanasios I.
Crosser, Orrin K.
Reed, X. B., Jr.
Hilgers, Michael Gene
Avula, Xavier J. R.
Chemical and Biochemical Engineering
Ph. D. in Chemical Engineering
University of Missouri--Rolla
Journal article titles appearing in thesis/dissertation
- "Perfusion chromatography": performance of periodic countercurrent column operation and its comparison with fixed bed operation
- Multi-component perfusion chromatography in fixed bed and periodic countercurrent operation
- The effects of structural and kinetic parameters on the performance of chromatographic columns packed with perfusive and purely diffusive adsorbent particles
- Estimation of pore diameter for intraparticle fluid flow in bidisperse porous chromatographic particles
- Affinity adsorption of adsorbates into spherical monodisperse and bidisperse porous perfusuve and purely diffusive adsorbent particles packed in a column: parameter estimation in the Laplace transform domain
- Model discrimination and estimation of the intraparticle mass transfer parameters for the adsorption of bovine serum albumin onto porous adsorbent particles using experimental frontal analysis data
xxx, 438 pages
© 1997 Glenn Allen Heeter, All rights reserved.
Dissertation - Restricted Access
Adsorption -- Mathematical models
Diffusion -- Mathematical models
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Electronic access to the full-text of this document is restricted to Missouri S&T users. Otherwise, request this publication directly from Missouri S&T Library or contact your local library.http://merlin.lib.umsystem.edu/record=b3643834~S5
Heeter, Glenn Allen, "The modelling and analysis of the dynamic performance of perfusion chromatography in fixed bed and periodic countercurrent column operations and parameter estimation and model discrimination using experimental data" (1997). Doctoral Dissertations. 1199.
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