Injection by Hydrostatic Pressure in Conjunction with Electrokinetic Force on a Microfluidic Chip
A simple method was developed for injecting a sample on a cross-form microfluidic chip by means of hydrostatic pressure combined with electrokinetic forces. The hydrostatic pressure was generated simply by adjusting the liquid level in different reservoirs without any additional driven equipment such as a pump. Two dispensing strategies using a floating injection and a gated injection, coupled with hydrostatic pressure loading, were tested. The fluorescence observation verified the feasibility of hydrostatic pressure loading in the separation of a mixture of fluorescein sodium salt and fluorescein isothiocyanate. This method was proved to be effective in leading cells to a separation channel for single cell analysis.
H. Gai et al., "Injection by Hydrostatic Pressure in Conjunction with Electrokinetic Force on a Microfluidic Chip," ELECTROPHORESIS, vol. 25, no. 12, pp. 1888-1894, John Wiley & Sons, Jun 2004.
The definitive version is available at https://doi.org/10.1002/elps.200305835
National Natural Science Foundation (China)
Keywords and Phrases
Fluorescein Isothiocyanate; Fluorescein Sodium; Analytic Method; Animal Cell; Cell Separation; Controlled Study; Device; Electric Potential; Equipment Design; Feasibility Study; Flow Kinetics; Fluid Flow; Fluorescence Analysis; Hydrostatic Pressure; Injection; Methodology; Microprocessor; Nonhuman; Pump; Separation Technique; Animals; Carps; Electromagnetic Fields; Electrophoresis, Microchip; Erythrocytes; Fluorescence; Hydrostatic Pressure; Microfluidics
International Standard Serial Number (ISSN)
Article - Journal
© 2004 John Wiley & Sons, All rights reserved.
01 Jun 2004