Abstract
This work reports the development and evaluation of dendrimer-based nanogels based on Poly amidoamine (PAMAM) dendrimer generation 5, engineered to act as a carrier with reactive oxygen species (ROS)-scavenging capabilities. We developed a cross-linking reaction-enabled flash nanoprecipitation method in which the cross-linking reaction occurs during the flash nanoprecipitation process to form a cross-linked nanostructure. Using this approach, an N-hydroxy succinimide (NHS)-functionalized ROS-responsive thioketal cross-linker (TK-NHS) was synthesized and utilized to cross-link DAB-core PAMAM dendrimer G5, resulting in the formation of G5-TK nanogels. The resulting nanogels were characterized using dynamic light scattering and transmission electron microscopy, and their cytocompatibility, irritancy, cellular uptake, and ROS scavenging activity were assessed. We confirmed the ROS scavenging capability of these nanogels and observed favorable safety profiles. The G5-TK nanogels can be further developed as carriers for therapeutic delivery applications to treat oxidative stress-related pathological conditions.
Recommended Citation
L. Qi et al., "Synthesis and Characterization of Free Radical Scavenging Dendrimer Nanogels via Cross-Linking Reaction-Enabled Flash Nanoprecipitation," Biomacromolecules, American Chemical Society, Jan 2025.
The definitive version is available at https://doi.org/10.1021/acs.biomac.5c00050
Department(s)
Chemistry
Second Department
Chemical and Biochemical Engineering
International Standard Serial Number (ISSN)
1526-4602; 1525-7797
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2025 American Chemical Society, All rights reserved.
Publication Date
01 Jan 2025
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Biomedical Engineering and Bioengineering Commons, Chemical Engineering Commons, Chemistry Commons
Comments
National Institutes of Health, Grant R01EY035088