Abstract
The use of enzyme-Based biosensors for the detection and quantification of analytes of interest such as contaminants of emerging concern, including over-the-counter medication, provides an attractive alternative compared to more established techniques. However, their direct application to real environmental matrices is still under investigation due to the various drawbacks in their implementation. Here, we report the development of bioelectrodes using laccase enzymes immobilized onto carbon paper electrodes modified with nanostructured molybdenum disulfide (MoS2). the laccase enzymes were two isoforms (LacI and LacII) produced and purified from the fungus Pycnoporus sanguineus CS43 that is native to Mexico. a commercial purified enzyme from the fungus Trametes versicolor (TvL) was also evaluated to compare their performance. the developed bioelectrodes were used in the biosensing of acetaminophen, a drug widely used to relieve fever and pain, and of which there is recent concern about its effect on the environment after its final disposal. the use of MoS2 as a transducer modifier was evaluated, and it was found that the best detection was achieved using a concentration of 1 mg/mL. Moreover, it was found that the laccase with the best biosensing efficiency was LacII, which achieved an LOD of 0.2 µM and a sensitivity of 0.108 µA/µM cm2 in the buffer matrix. Moreover, the performance of the bioelectrodes in a composite groundwater sample from Northeast Mexico was analyzed, achieving an LOD of 0.5 µM and a sensitivity of 0.015 µA/µM cm2. the LOD values found are among the lowest reported for biosensors based on the use of oxidoreductase enzymes, while the sensitivity is the highest currently reported.
Recommended Citation
M. Herrera-Domínguez et al., "Detection of Acetaminophen in Groundwater by Laccase-Based Amperometric Biosensors using MoS2 Modified Carbon Paper Electrodes," Sensors, vol. 23, no. 10, article no. 4633, MDPI, May 2023.
The definitive version is available at https://doi.org/10.3390/s23104633
Department(s)
Chemistry
Publication Status
Open Access
Keywords and Phrases
acetaminophen; electrochemical biosensor; emerging pollutants; laccases; MoS 2
International Standard Serial Number (ISSN)
1424-8220
Document Type
Article - Journal
Document Version
Final Version
File Type
text
Language(s)
English
Rights
© 2025 The Authors, All rights reserved.
Creative Commons Licensing
This work is licensed under a Creative Commons Attribution 4.0 License.
Publication Date
01 May 2023
PubMed ID
37430547
Comments
Consejo Nacional de Ciencia y Tecnología, Grant -2016-4320