Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells
Previous studies have shown that chemotherapeutic efficacy could be enhanced with targeted drug delivery. Various DNA origami nanostructures have been investigated as drug carriers. Here, we compared drug delivery functionalities of three similar DNA origami nanostructures, Disc, Donut, and Sphere, that differ in structural dimension. Our results demonstrated that Donut was the most stable and exhibited the highest Dox-loading capacity. MUC1 aptamer modification in our nanostructures increased cellular uptake in MUC1-high MCF-7. Among the three nanostructures, unmodified Donut exerted the highest Dox cytotoxicity in MCF-7, and MUC1 aptamer modification did not further improve its effect, implicating that Dox delivery by Donut was efficient. However, all Dox-loaded nanostructures showed comparable cytotoxicity in MDA-MB-231 due to the innate sensitivity of this cell line to Dox. Our results successfully demonstrated that functional properties of DNA origami nanocarriers could be tuned by structural design, and three-dimensional Donut appeared to be the most efficient nanocarrier.
A. Udomprasert and C. Wootthichairangsan and R. Duangrat and S. Chaithongyot and Y. Zhang and R. Nixon and W. Liu and R. Wang and M. Ponglikitmongkol and T. Kangsamaksin, "Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells," ACS Applied Bio Materials, American Chemical Society, Jan 2022.
The definitive version is available at https://doi.org/10.1021/acsabm.2c00114
Keywords and Phrases
DNA Origami Nanostructures; Doxorubicin; Drug Delivery System; Enhanced Specificity; MUC1 Aptamer
International Standard Serial Number (ISSN)
Article - Journal
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01 Jan 2022