Folic Acid-Decorated Polyamidoamine Dendrimer Mediates Selective Uptake and High Expression of Genes in Head and Neck Cancer Cells
Aim: Folic acid (FA)-decorated polyamidoamine dendrimer G4 (G4-FA) was synthesized and studied for targeted delivery of genes to head and neck cancer cells expressing high levels of folate receptors (FRs). Methods: Cellular uptake, targeting specificity, cytocompatibility and transfection efficiency were evaluated. Results: G4-FA competes with free FA for the same binding site. G4-FA facilitates the cellular uptake of DNA plasmids in a FR-dependent manner and selectively delivers plasmids to FR-high cells, leading to enhanced gene expression. Conclusion: G4-FA is a suitable vector to deliver genes selectively to head and neck cancer cells. The fundamental understandings of G4-FA as a vector and its encouraging transfection results for head and neck cancer cells provided support for its further testing in vivo.
L. Xu et al., "Folic Acid-Decorated Polyamidoamine Dendrimer Mediates Selective Uptake and High Expression of Genes in Head and Neck Cancer Cells," Nanomedicine, vol. 11, no. 22, pp. 2959-2973, Future Medicine Ltd., Nov 2016.
The definitive version is available at https://doi.org/10.2217/nnm-2016-0244
Chemical and Biochemical Engineering
Keywords and Phrases
folate receptor targeting; gene therapy; head and neck squamous cell carcinoma; PAMAM dendrimer
International Standard Serial Number (ISSN)
Article - Journal
© 2016 Future Medicine Ltd., All rights reserved.
01 Nov 2016