Title

Folic Acid-Decorated Polyamidoamine Dendrimer Mediates Selective Uptake and High Expression of Genes in Head and Neck Cancer Cells

Abstract

Aim: Folic acid (FA)-decorated polyamidoamine dendrimer G4 (G4-FA) was synthesized and studied for targeted delivery of genes to head and neck cancer cells expressing high levels of folate receptors (FRs). Methods: Cellular uptake, targeting specificity, cytocompatibility and transfection efficiency were evaluated. Results: G4-FA competes with free FA for the same binding site. G4-FA facilitates the cellular uptake of DNA plasmids in a FR-dependent manner and selectively delivers plasmids to FR-high cells, leading to enhanced gene expression. Conclusion: G4-FA is a suitable vector to deliver genes selectively to head and neck cancer cells. The fundamental understandings of G4-FA as a vector and its encouraging transfection results for head and neck cancer cells provided support for its further testing in vivo.

Department(s)

Chemical and Biochemical Engineering

Comments

This work was supported, in part, by the National Science Foundation (CAREER award CBET0954957), and National Institutes of Health (R01EY024072 and R01DE024381).

Keywords and Phrases

folate receptor targeting; gene therapy; head and neck squamous cell carcinoma; PAMAM dendrimer

International Standard Serial Number (ISSN)

1743-5889; 1748-6963

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2016 Future Medicine Ltd., All rights reserved.

Publication Date

01 Nov 2016

PubMed ID

27781559

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