Analysis of the Second Virial Coefficient of Glycosylated Proteins by Light Scattering
Department
Chemical and Biochemical Engineering
Major
Chemical Engineering
Research Advisor
Forciniti, Daniel
Advisor's Department
Chemical and Biochemical Engineering
Funding Source
Missouri S& T Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
The second virial coefficient, A2, has been used by others to characterize the stability of a protein in solution to screen for proper crystallization conditions. However, the effect of protein glycosylation on A2 has not been investigated. Ribonuclease A and B are isoforms of the same protein that differ by a single glycosylation. Solutions of Ribonuclease A were analyzed by light scattering at two pH and temperature levels. A2 was then determined for each condition by Zimm Plot analysis. Ribonuclease B was not tested yet. In future studies, A2 for Ribonuclease B will be measured and compared to the determined values of Ribonuclease A to determine the effect of glycosylation patterns on A2.
Biography
Sam is from Pontoon Beach, IL, is a senior in Chemical Engineering. He is the Vice-President of Missouri S& T's Academic Competition Organization and staffs high school Scholar Bowl tournaments throughout Missouri. He will graduate in May, 2013.
Research Category
Engineering
Presentation Type
Oral Presentation
Document Type
Presentation
Award
Engineering oral presentation, First place
Location
Carver Room
Presentation Date
03 Apr 2013, 10:30 am - 11:00 am
Analysis of the Second Virial Coefficient of Glycosylated Proteins by Light Scattering
Carver Room
The second virial coefficient, A2, has been used by others to characterize the stability of a protein in solution to screen for proper crystallization conditions. However, the effect of protein glycosylation on A2 has not been investigated. Ribonuclease A and B are isoforms of the same protein that differ by a single glycosylation. Solutions of Ribonuclease A were analyzed by light scattering at two pH and temperature levels. A2 was then determined for each condition by Zimm Plot analysis. Ribonuclease B was not tested yet. In future studies, A2 for Ribonuclease B will be measured and compared to the determined values of Ribonuclease A to determine the effect of glycosylation patterns on A2.
