Development of Portable Biosensor for Quantitative analysis of Tumor-Liberated Proteins in Human Serum
Department
Chemistry
Research Advisor
Ma, Yinfa
Advisor's Department
Chemistry
Abstract
The need for a quick, accurate and cost-effective method of determining the presence or stage of a tumor in a patient has led researchers to seek a new breed of instruments capable of meeting this demand. These instruments, commonly known as biosensors, are capable of detecting minute quantities of a selected proteomic target in solution or via any alternative method that allows uniform distribution of the proteomic target across the entire medium. One alternative method employed in this project involves the adsorption of the protein to a gold surface for measurement using fluorescent probes.
The project for this year was divided into two segments: research of previous projects similar in concept and design, as well as TLPs specific to non-small cell lung carcinoma (NSCLC) as potential targets for future study, and creation of the biosensor with preliminary testing.
Results suggest the use of a cytokeratin fragment, Cyfra 21-1, as a biomarker for monitoring the progress of tumor progression once diagnosis has been made. The biosensor also seems to show great promise for more studies involving similar methods.
Biography
Christopher Thomas Campbell is a sophomore attending the University of Missouri - Rolla to study biochemistry. He is the first son of Steven and Kimberly Campbell, who reside in Nixa, Missouri with his two younger brothers, Brian and Michael.
Research Category
Natural Sciences
Presentation Type
Oral Presentation
Document Type
Presentation
Award
Natural Sciences oral presentation, Thirid place
Presentation Date
12 Apr 2006, 9:00 am
Development of Portable Biosensor for Quantitative analysis of Tumor-Liberated Proteins in Human Serum
The need for a quick, accurate and cost-effective method of determining the presence or stage of a tumor in a patient has led researchers to seek a new breed of instruments capable of meeting this demand. These instruments, commonly known as biosensors, are capable of detecting minute quantities of a selected proteomic target in solution or via any alternative method that allows uniform distribution of the proteomic target across the entire medium. One alternative method employed in this project involves the adsorption of the protein to a gold surface for measurement using fluorescent probes.
The project for this year was divided into two segments: research of previous projects similar in concept and design, as well as TLPs specific to non-small cell lung carcinoma (NSCLC) as potential targets for future study, and creation of the biosensor with preliminary testing.
Results suggest the use of a cytokeratin fragment, Cyfra 21-1, as a biomarker for monitoring the progress of tumor progression once diagnosis has been made. The biosensor also seems to show great promise for more studies involving similar methods.
