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| Title: | Visualizing chemical interactions in life sciences with wide-field fluorescence microscopy towards the single-molecule level |
| Author (s): | Gai, Hongwei Stayton, Isaac Liu, Xin Lin, Bingcheng Ma, Yinfa |
| Department/Lab Affiliations: | Chemistry Environmental Research Center |
| Keywords: | Interaction. Single-molecule imaging. Wide-field microscopy. |
| Subject Terms: | Fluorescence. |
| Issue Date: | 2007 |
| Publisher: | Elsevier |
| Citation: | Gai, Hongweii, Stayton, Isaac, Liu, Xin, Lin, Bingcheng, and Ma, Yinfa, “Visualizing chemical interactions in life sciences with wide-field fluorescence microscopy towards the single molecule level: A minireview", Trends in Analytical Chemistry, Volume 26, Issue 10, November 2007, Pages 980-992 |
| Abstract: | Systematic understanding of dynamic biological networks is grounded at the molecular level. Knowledge at this level will discover the functions of molecular components and their interactions with cells or among the molecules themselves. Single-molecule detection has proved to be an indispensable technique for characterizing molecular interactions in various physiological conditions. Among the single-molecule-detection methods, wide-field microscopy-based fluorescence imaging is unique in that dynamic single-molecule events can be vividly observed in a wide observation field. Such a technique has obvious advantages for direct tracking of the pathways and processes of molecular interactions and giving visual information about the molecular mechanisms of many biological events. We discuss experimental schemes and applications of wide-field fluorescence microscopy in characterizing biological molecular interactions and studying virus-cell or particle-cell interactions. |
| Type: | Article - Journal text |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. FULL COPYRIGHT INFORMATION: |
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| title | Visualizing chemical interactions in life sciences with wide-field fluorescence microscopy towards the single-molecule level |
| contributor.author | Gai, Hongwei |
| contributor.author | Stayton, Isaac |
| contributor.author | Liu, Xin |
| contributor.author | Lin, Bingcheng |
| contributor.author | Ma, Yinfa |
| contributor.deptlab | Chemistry |
| contributor.deptlab | Environmental Research Center |
| subject | Interaction. |
| subject | Single-molecule imaging. |
| subject | Wide-field microscopy. |
| subject.LCSH | Fluorescence. |
| date.issued | 2007 |
| publisher | Elsevier |
| identifier.citation | Gai, Hongweii, Stayton, Isaac, Liu, Xin, Lin, Bingcheng, and Ma, Yinfa, “Visualizing chemical interactions in life sciences with wide-field fluorescence microscopy towards the single molecule level: A minireview", Trends in Analytical Chemistry, Volume 26, Issue 10, November 2007, Pages 980-992 |
| identifier.pub.URI | |
| description.abstract | Systematic understanding of dynamic biological networks is grounded at the molecular level. Knowledge at this level will discover the functions of molecular components and their interactions with cells or among the molecules themselves. Single-molecule detection has proved to be an indispensable technique for characterizing molecular interactions in various physiological conditions. Among the single-molecule-detection methods, wide-field microscopy-based fluorescence imaging is unique in that dynamic single-molecule events can be vividly observed in a wide observation field. Such a technique has obvious advantages for direct tracking of the pathways and processes of molecular interactions and giving visual information about the molecular mechanisms of many biological events. We discuss experimental schemes and applications of wide-field fluorescence microscopy in characterizing biological molecular interactions and studying virus-cell or particle-cell interactions. |
| type | Article - Journal |
| type.DCMIType | text |
| type.status | Final version |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. |
| rights.URI | |
| date.accessioned | 2007-04-11T17:00:48Z |
| date.available | 2007-12-17T21:02:33Z |
| identifier.persist.URI |