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| Title: | Toxicity of nano- and micro-sized ZnO particles in human lung epithelial cells |
| Author (s): | Lin, Weisheng Xu, Yi Huang, Chuan-Chin Ma, Yinfa Shannon, Katie Chen, Da-Ren Huang, Yue-wern |
| Department/Lab Affiliations: | Biological Sciences Center for Environmental Science and Technology (CEST) Chemistry Environmental Research Center |
| Keywords: | EHS ZnO human bronchoalveolar carcinoma-derived cell (A549) lipid peroxidation nanotechnology occupational health oxidative DNA damage oxidative stress particles |
| Issue Date: | 2009-01 |
| Publisher: | Springer Verlag |
| Citation: | Lin, Weisheng, Yi Xu, Chuan-Chin Huang, Yinfa Ma, Katie B. Shannon, Da-Ren Chen and Yue-Wern Huang. "Toxicity of nano- and micro-sized ZnO particles in human lung epithelial cells." Journal of Nanoparticle Research, Vol 11 No 1, January 2009: 25-39. |
| Abstract: | This is the first comprehensive study to evaluate the cytotoxicity, biochemical mechanisms of toxicity, and oxidative DNA damage caused by exposing human bronchoalveolar carcinoma-derived cells (A549) to 70 and 420 nm ZnO particles. Particles of either size significantly reduced cell viability in a dose- and time-dependent manner within a rather narrow dosage range. Particle mass-based dosimetry and particle-specific surface area-based dosimetry yielded two distinct patterns of cytotoxicity in both 70 and 420 nm ZnO particles. Elevated levels of reactive oxygen species (ROS) resulted in intracellular oxidative stress, lipid peroxidation, cell membrane leakage, and oxidative DNA damage. The protective effect of N-acetylcysteine on ZnO-induced cytotoxicity further implicated oxidative stress in the cytotoxicity. Free Zn2+ and metal impurities were not major contributors of ROS induction as indicated by limited free Zn2+ cytotoxicity, extent of Zn2+ dissociation in the cell culture medium, and inductively-coupled plasma-mass spectrometry metal analysis. We conclude that (1) exposure to both sizes of ZnO particles leads to dose- and time-dependent cytotoxicity reflected in oxidative stress, lipid peroxidation, cell membrane damage, and oxidative DNA damage, (2) ZnO particles exhibit a much steeper dose–response pattern unseen in other metal oxides, and (3) neither free Zn2+ nor metal impurity in the ZnO particle samples is the cause of cytotoxicity. |
| Type: | Article - Journal text |
| In Title: | Journal of Nanoparticle Research |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. Pre-print: author can archive; Post-print: author can archive; FULL COPYRIGHT INFORMATION: |
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| title | Toxicity of nano- and micro-sized ZnO particles in human lung epithelial cells |
| contributor.author | Lin, Weisheng |
| contributor.author | Xu, Yi |
| contributor.author | Huang, Chuan-Chin |
| contributor.author | Ma, Yinfa |
| contributor.author | Shannon, Katie |
| contributor.author | Chen, Da-Ren |
| contributor.author | Huang, Yue-wern |
| contributor.deptlab | Biological Sciences |
| contributor.deptlab | Center for Environmental Science and Technology (CEST) |
| contributor.deptlab | Chemistry |
| contributor.deptlab | Environmental Research Center |
| contributor.sponsor | CDNA Resource Center |
| contributor.sponsor | Environmental Research Center at the Missouri University of Science and Technology |
| subject | EHS |
| subject | ZnO |
| subject | human bronchoalveolar carcinoma-derived cell (A549) |
| subject | lipid peroxidation |
| subject | nanotechnology |
| subject | occupational health |
| subject | oxidative DNA damage |
| subject | oxidative stress |
| subject | particles |
| date.issued | 2009-01 |
| publisher | Springer Verlag |
| identifier.citation | Lin, Weisheng, Yi Xu, Chuan-Chin Huang, Yinfa Ma, Katie B. Shannon, Da-Ren Chen and Yue-Wern Huang. "Toxicity of nano- and micro-sized ZnO particles in human lung epithelial cells." Journal of Nanoparticle Research, Vol 11 No 1, January 2009: 25-39. |
| identifier.pub.URI | |
| description.abstract | This is the first comprehensive study to evaluate the cytotoxicity, biochemical mechanisms of toxicity, and oxidative DNA damage caused by exposing human bronchoalveolar carcinoma-derived cells (A549) to 70 and 420 nm ZnO particles. Particles of either size significantly reduced cell viability in a dose- and time-dependent manner within a rather narrow dosage range. Particle mass-based dosimetry and particle-specific surface area-based dosimetry yielded two distinct patterns of cytotoxicity in both 70 and 420 nm ZnO particles. Elevated levels of reactive oxygen species (ROS) resulted in intracellular oxidative stress, lipid peroxidation, cell membrane leakage, and oxidative DNA damage. The protective effect of N-acetylcysteine on ZnO-induced cytotoxicity further implicated oxidative stress in the cytotoxicity. Free Zn2+ and metal impurities were not major contributors of ROS induction as indicated by limited free Zn2+ cytotoxicity, extent of Zn2+ dissociation in the cell culture medium, and inductively-coupled plasma-mass spectrometry metal analysis. We conclude that (1) exposure to both sizes of ZnO particles leads to dose- and time-dependent cytotoxicity reflected in oxidative stress, lipid peroxidation, cell membrane damage, and oxidative DNA damage, (2) ZnO particles exhibit a much steeper dose–response pattern unseen in other metal oxides, and (3) neither free Zn2+ nor metal impurity in the ZnO particle samples is the cause of cytotoxicity. |
| type | Article - Journal |
| type.DCMIType | text |
| type.status | Postprint |
| relation.isPartOf | Journal of Nanoparticle Research |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. |
| rights | Pre-print: author can archive; Post-print: author can archive; |
| rights.URI | |
| rights.URI | |
| identifier.persist.URI | |
| date.available | 2009-01-13T17:27:23Z |