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| Title: | Spatial and temporal patterns in the microbial diversity of a meromictic soda lake in Washington State |
| Author (s): | Dimitriu, Pedro A. Pinkart, Holly C. Peyton, Brent M. Mormile, Melanie R. |
| Department/Lab Affiliations: | Biological Sciences Energy Research and Development Center Environmental Research Center |
| Keywords: | Meromictic |
| Subject Terms: | Microbial diversity. Soap Lake (Grant County, Wash. : Lake) |
| Issue Date: | 2008-08 |
| Publisher: | American Society for Microbiology |
| Citation: | Dimitriu, Pedro A., Holly C. Pinkart, Brent M. Peyton, and Melanie R. Mormile. “Microbial diversity of a meromictic soda lake in Washington State”, Applied and Environmental Microbiology, Vol. 74, No. 15, 2008, pp. 4877-4888. |
| Abstract: | The microbial community diversity and composition of meromictic Soap Lake were studied using culture-dependent and culture-independent approaches. The water column and sediments were sampled monthly for a year. Denaturing gradient gel electrophoresis of bacterial and archaeal 16S rRNA genes showed an increase in diversity with depth for both groups. Late-summer samples harbored the highest prokaryotic diversity, and the bacteria exhibited less seasonal variability than the archaea. Most-probable-number assays targeting anaerobic microbial guilds were performed to compare summer and fall samples. In both seasons, the anoxic samples appeared to be dominated by lactate-oxidizing sulfate-reducing prokaryotes. High numbers of lactate- and acetate-oxidizing iron-reducing bacteria, as well as fermentative microorganisms, were also found, whereas the numbers of methanogens were low or methanogens were undetectable. The bacterial community composition of summer and fall samples was also assessed by constructing 16S rRNA gene clone libraries. A total of 508 sequences represented an estimated >1,100 unique operational taxonomic units, most of which were from the monimolimnion, and the summer samples were more diverse than the fall samples (Chao1 = 530 and Chao1 = 295, respectively). For both seasons, the mixolimnion sequences were dominated by Gammaproteobacteria, and the chemocline and monimolimnion libraries were dominated by members of the low-G+C-content group, followed by the Cytophaga-Flexibacter-Bacteroides (CFB) group; the mixolimnion sediments contained sequences related to uncultured members of the Chloroflexi and the CFB group. Community overlap and phylogenetic analyses, however, not only demonstrated that there was a high degree of spatial turnover but also suggested that there was a degree of temporal variability due to differences in the members and structures of the communities. |
| Type: | Article - Journal text |
| In Title: | Applied and Environmental Microbiology |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. Pre-print: author cannot archive; Post-print: author can archive; FULL COPYRIGHT INFORMATION: |
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| title | Spatial and temporal patterns in the microbial diversity of a meromictic soda lake in Washington State |
| contributor.author | Dimitriu, Pedro A. |
| contributor.author | Pinkart, Holly C. |
| contributor.author | Peyton, Brent M. |
| contributor.author | Mormile, Melanie R. |
| contributor.deptlab | Biological Sciences |
| contributor.deptlab | Energy Research and Development Center |
| contributor.deptlab | Environmental Research Center |
| contributor.sponsor | Microbial Observatories Program of the United States National Science Foundation |
| subject | Meromictic |
| subject.LCSH | Microbial diversity. |
| subject.LCSH | Soap Lake (Grant County, Wash. : Lake) |
| date.issued | 2008-08 |
| publisher | American Society for Microbiology |
| identifier.citation | Dimitriu, Pedro A., Holly C. Pinkart, Brent M. Peyton, and Melanie R. Mormile. “Microbial diversity of a meromictic soda lake in Washington State”, Applied and Environmental Microbiology, Vol. 74, No. 15, 2008, pp. 4877-4888. |
| identifier.pub.URI | |
| description.abstract | The microbial community diversity and composition of meromictic Soap Lake were studied using culture-dependent and culture-independent approaches. The water column and sediments were sampled monthly for a year. Denaturing gradient gel electrophoresis of bacterial and archaeal 16S rRNA genes showed an increase in diversity with depth for both groups. Late-summer samples harbored the highest prokaryotic diversity, and the bacteria exhibited less seasonal variability than the archaea. Most-probable-number assays targeting anaerobic microbial guilds were performed to compare summer and fall samples. In both seasons, the anoxic samples appeared to be dominated by lactate-oxidizing sulfate-reducing prokaryotes. High numbers of lactate- and acetate-oxidizing iron-reducing bacteria, as well as fermentative microorganisms, were also found, whereas the numbers of methanogens were low or methanogens were undetectable. The bacterial community composition of summer and fall samples was also assessed by constructing 16S rRNA gene clone libraries. A total of 508 sequences represented an estimated >1,100 unique operational taxonomic units, most of which were from the monimolimnion, and the summer samples were more diverse than the fall samples (Chao1 = 530 and Chao1 = 295, respectively). For both seasons, the mixolimnion sequences were dominated by Gammaproteobacteria, and the chemocline and monimolimnion libraries were dominated by members of the low-G+C-content group, followed by the Cytophaga-Flexibacter-Bacteroides (CFB) group; the mixolimnion sediments contained sequences related to uncultured members of the Chloroflexi and the CFB group. Community overlap and phylogenetic analyses, however, not only demonstrated that there was a high degree of spatial turnover but also suggested that there was a degree of temporal variability due to differences in the members and structures of the communities. |
| type | Article - Journal |
| type.DCMIType | text |
| type.status | Postprint |
| relation.isPartOf | Applied and Environmental Microbiology |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. |
| rights | Pre-print: author cannot archive; Post-print: author can archive; |
| identifier.persist.URI | |
| date.available | 2009-01-05T22:43:00Z |