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| Title: | In vitro toxicity of silica nanoparticles in human lung cancer cells |
| Author (s): | Lin, Weisheng Huang, Yue-wern Zhou, Xiao-Dong Ma, Yinfa |
| Department/Lab Affiliations: | Biological Sciences Chemistry Environmental Research Center |
| Keywords: | Cytotoxicity. Lung cancer cells (A549). |
| Subject Terms: | Nanoparticles. Oxidative stress. Silica. |
| Issue Date: | 2006 |
| Publisher: | Elsevier |
| Citation: | Lin, Weisheng, Huang, Yue-wern, Zhou, Xiao-Dong, and Ma, Yinfa. 2006. In Vitro toxicity of silica nanoparticles in human lung cancer cells. Toxicology and Applied Pharmacology 217: 252-259. |
| Abstract: | The cytotoxicity of 15-nm and 46-nm silica nanoparticles was investigated by using crystalline silica (Min-U-Sil 5) as a positive control in cultured human bronchoalveolar carcinoma-derived cells. Exposure to 15-nm or 46-nm SiO2 nanoparticles for 48 h at dosage levels between 10 and 100 μg/ml decreased cell viability in a dose-dependent manner. Both SiO2 nanoparticles were more cytotoxic than Min-U-Sil 5; however, the cytotoxicities of 15-nm and 46-nm silica nanoparticles were not significantly different. The 15-nm SiO2 nanoparticles were used to determine time-dependent cytotoxicity and oxidative stress responses. Cell viability decreased significantly as a function of both nanoparticle dosage (10–100 μg/ml) and exposure time (24 h, 48 h, and 72 h). Indicators of oxidative stress and cytotoxicity, including total reactive oxygen species (ROS), glutathione, malondialdehyde, and lactate dehydrogenase, were quantitatively assessed. Exposure to SiO2 nanoparticles increased ROS levels and reduced glutathione levels. The increased production of malondialdehyde and lactate dehydrogenase release from the cells indicated lipid peroxidation and membrane damage. In summary, exposure to SiO2 nanoparticles results in a dose-dependent cytotoxicity in cultural human bronchoalveolar carcinoma-derived cells that is closely correlated to increased oxidative stress. |
| Type: | Article - Journal text |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. FULL COPYRIGHT INFORMATION: |
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| title | In vitro toxicity of silica nanoparticles in human lung cancer cells |
| contributor.author | Lin, Weisheng |
| contributor.author | Huang, Yue-wern |
| contributor.author | Zhou, Xiao-Dong |
| contributor.author | Ma, Yinfa |
| contributor.deptlab | Biological Sciences |
| contributor.deptlab | Chemistry |
| contributor.deptlab | Environmental Research Center |
| subject | Cytotoxicity. |
| subject | Lung cancer cells (A549). |
| subject.LCSH | Nanoparticles. |
| subject.LCSH | Oxidative stress. |
| subject.LCSH | Silica. |
| date.issued | 2006 |
| publisher | Elsevier |
| identifier.citation | Lin, Weisheng, Huang, Yue-wern, Zhou, Xiao-Dong, and Ma, Yinfa. 2006. In Vitro toxicity of silica nanoparticles in human lung cancer cells. Toxicology and Applied Pharmacology 217: 252-259. |
| identifier.pub.URI | |
| description.abstract | The cytotoxicity of 15-nm and 46-nm silica nanoparticles was investigated by using crystalline silica (Min-U-Sil 5) as a positive control in cultured human bronchoalveolar carcinoma-derived cells. Exposure to 15-nm or 46-nm SiO2 nanoparticles for 48 h at dosage levels between 10 and 100 μg/ml decreased cell viability in a dose-dependent manner. Both SiO2 nanoparticles were more cytotoxic than Min-U-Sil 5; however, the cytotoxicities of 15-nm and 46-nm silica nanoparticles were not significantly different. The 15-nm SiO2 nanoparticles were used to determine time-dependent cytotoxicity and oxidative stress responses. Cell viability decreased significantly as a function of both nanoparticle dosage (10–100 μg/ml) and exposure time (24 h, 48 h, and 72 h). Indicators of oxidative stress and cytotoxicity, including total reactive oxygen species (ROS), glutathione, malondialdehyde, and lactate dehydrogenase, were quantitatively assessed. Exposure to SiO2 nanoparticles increased ROS levels and reduced glutathione levels. The increased production of malondialdehyde and lactate dehydrogenase release from the cells indicated lipid peroxidation and membrane damage. In summary, exposure to SiO2 nanoparticles results in a dose-dependent cytotoxicity in cultural human bronchoalveolar carcinoma-derived cells that is closely correlated to increased oxidative stress. |
| type | Article - Journal |
| type.DCMIType | text |
| type.status | Final version |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. |
| rights.URI | |
| date.accessioned | 2007-04-11T17:00:48Z |
| date.available | 2007-12-17T20:49:13Z |
| identifier.persist.URI |