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| Title: | High performance liquid chromatography analysis of 2-mercaptoethylamine (cysteamine) in biological samples by derivatization with N-(1-pyrenyl) maleimide (NPM) using fluorescence detection |
| Author (s): | Ogony, J. Mare, S. Wu, W. Ercal, Nuran |
| Department/Lab Affiliations: | Chemistry Environmental Research Center |
| Keywords: | 2-Mercaptoethylamine. CSH. Cysteamine. NPM. |
| Subject Terms: | Cystinosis. High performance liquid chromatography. Oxidative stress. Thiols. |
| Issue Date: | 2006 |
| Publisher: | Elsevier |
| Citation: | Ogony, J., Mare, S., Wu, W., and Ercal, N. “High Performance Liquid Chromatography Analysis of 2-Mercaptoethylamine (Cysteamine) in Biological Samples by Derivatization with N-(1-pyrenyl) maleimide (NPM) using Fluorescence Detection,” Biomedical Chromatography,Volume 843, Issue 1, 20 October 2006, Pages 57-62 2006. |
| Abstract: | 2-Mercaptoethylamine (cysteamine) is an aminothiol compound used as a drug for the treatment of cystinosis, an autosomal recessive lysosomal storage disorder. Because of cysteamine's important role in clinical settings, its analysis by sensitive techniques has become pivotal. Unfortunately, the available methods are either complex or labor intensive. Therefore, we have developed a new rapid, sensitive, and simple method for determining cysteamine in biological samples (brain, kidney, liver, and plasma), using N-(1-pyrenyl) maleimide (NPM) as the derivatizing agent and reversed-phase high performance liquid chromatography (HPLC) with a fluorescence detection method (λex = 330 nm, λem = 376 nm). The mobile phase was acetonitrile and water (70:30) with acetic acid and o-phosphoric acid (1 mL/L). The calibration curve for cysteamine in serine borate buffer (SBB) was found to be linear over a range of 0–1200 nM (r2 = 0.9993), and in plasma and liver matrix, the r2 values were 0.9968 and 0.9965, respectively. The coefficients of the variation for the within-run and between-run precisions ranged from 0.68 to 9.90% and 0.63 to 4.17%, respectively. The percentage of relative recovery ranged from 94.1 to 98.6%. |
| Type: | Article - Journal text |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. FULL COPYRIGHT INFORMATION: |
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| title | High performance liquid chromatography analysis of 2-mercaptoethylamine (cysteamine) in biological samples by derivatization with N-(1-pyrenyl) maleimide (NPM) using fluorescence detection |
| contributor.author | Ogony, J. |
| contributor.author | Mare, S. |
| contributor.author | Wu, W. |
| contributor.author | Ercal, Nuran |
| contributor.deptlab | Chemistry |
| contributor.deptlab | Environmental Research Center |
| subject | 2-Mercaptoethylamine. |
| subject | CSH. |
| subject | Cysteamine. |
| subject | NPM. |
| subject.LCSH | Cystinosis. |
| subject.LCSH | High performance liquid chromatography. |
| subject.LCSH | Oxidative stress. |
| subject.LCSH | Thiols. |
| date.issued | 2006 |
| publisher | Elsevier |
| identifier.citation | Ogony, J., Mare, S., Wu, W., and Ercal, N. “High Performance Liquid Chromatography Analysis of 2-Mercaptoethylamine (Cysteamine) in Biological Samples by Derivatization with N-(1-pyrenyl) maleimide (NPM) using Fluorescence Detection,” Biomedical Chromatography,Volume 843, Issue 1, 20 October 2006, Pages 57-62 2006. |
| identifier.pub.URI | |
| description.abstract | 2-Mercaptoethylamine (cysteamine) is an aminothiol compound used as a drug for the treatment of cystinosis, an autosomal recessive lysosomal storage disorder. Because of cysteamine's important role in clinical settings, its analysis by sensitive techniques has become pivotal. Unfortunately, the available methods are either complex or labor intensive. Therefore, we have developed a new rapid, sensitive, and simple method for determining cysteamine in biological samples (brain, kidney, liver, and plasma), using N-(1-pyrenyl) maleimide (NPM) as the derivatizing agent and reversed-phase high performance liquid chromatography (HPLC) with a fluorescence detection method (λex = 330 nm, λem = 376 nm). The mobile phase was acetonitrile and water (70:30) with acetic acid and o-phosphoric acid (1 mL/L). The calibration curve for cysteamine in serine borate buffer (SBB) was found to be linear over a range of 0–1200 nM (r2 = 0.9993), and in plasma and liver matrix, the r2 values were 0.9968 and 0.9965, respectively. The coefficients of the variation for the within-run and between-run precisions ranged from 0.68 to 9.90% and 0.63 to 4.17%, respectively. The percentage of relative recovery ranged from 94.1 to 98.6%. |
| type | Article - Journal |
| type.DCMIType | text |
| type.status | Final version |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. |
| rights.URI | |
| date.accessioned | 2007-04-11T17:00:48Z |
| date.available | 2007-12-17T20:47:59Z |
| identifier.persist.URI |