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| Title: | Evaluation of hydroxyapatite microspheres made from a borate glass to separate protein mixtures |
| Author (s): | Han, Xue Du, Ming Ma, Yinfa Day, Delbert E. |
| Department/Lab Affiliations: | Center for Environmental Science and Technology (CEST) Chemistry Environmental Research Center Materials Research Center Materials Science & Engineering |
| Keywords: | borate glass |
| Subject Terms: | Hydroxyapatite. Protein - separation |
| Issue Date: | 2008-08 |
| Publisher: | Springer Verlag |
| Citation: | Han, Xue , Ming Du, Yinfa Ma, and Delbert E. Day. "Evaluation of hydroxyapatite microspheres made from a borate glass to separate protein mixtures." Journal of Material Science, Volume 43, Number 16 , (August, 2008): 5618-5625. |
| Abstract: | A hydroxyapatite (HA, Ca10(PO4)6(OH)2), transformed from a calcium-containing borate glass, has been investigated for its protein adsorption and chromatographic characteristics. Microspheres of the borate glass were transformed into HA by reacting them with a 0.25 M phosphate (K2HPO4) solution for 24 h at 37 °C (pH 9.0). The HA microspheres with a diameter of 45–90 μm were hand packed into a steel column (4.6 mm × 80 mm) and used to separate a binary protein mixture of bovine serum albumin (BSA) and lysozyme. HA microspheres, with a diameter <45 μm, were used for separating a protein mixture of BSA, myoglobin, and lysozyme. These microspheres had a diameter that was 20–30 times larger than commercial HA column packing spherical particles, 2–3 μm, but these microspheres had a six times larger surface area and a more uniform spherical shape. These advantages compensated for their larger size and the separation results were comparable to those commercially available HA columns in the separation of the proteins studied. These unique HA microspheres, made from microspheres of a borate glass, are considered to be useful as packing materials for protein separation in chromatography. |
| Type: | Article - Journal text |
| In Title: | Journal of Materials Science |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. Pre-print: author can archive; Post-print: author can archive; FULL COPYRIGHT INFORMATION: |
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| title | Evaluation of hydroxyapatite microspheres made from a borate glass to separate protein mixtures |
| contributor.author | Han, Xue |
| contributor.author | Du, Ming |
| contributor.author | Ma, Yinfa |
| contributor.author | Day, Delbert E. |
| contributor.deptlab | Center for Environmental Science and Technology (CEST) |
| contributor.deptlab | Chemistry |
| contributor.deptlab | Environmental Research Center |
| contributor.deptlab | Materials Research Center |
| contributor.deptlab | Materials Science & Engineering |
| contributor.sponsor | Materials Research Center at the University of Missouri - Rolla |
| subject | borate glass |
| subject.LCSH | Hydroxyapatite. |
| subject.LCSH | Protein - separation |
| date.issued | 2008-08 |
| publisher | Springer Verlag |
| identifier.citation | Han, Xue , Ming Du, Yinfa Ma, and Delbert E. Day. "Evaluation of hydroxyapatite microspheres made from a borate glass to separate protein mixtures." Journal of Material Science, Volume 43, Number 16 , (August, 2008): 5618-5625. |
| identifier.pub.URI | |
| description.abstract | A hydroxyapatite (HA, Ca10(PO4)6(OH)2), transformed from a calcium-containing borate glass, has been investigated for its protein adsorption and chromatographic characteristics. Microspheres of the borate glass were transformed into HA by reacting them with a 0.25 M phosphate (K2HPO4) solution for 24 h at 37 °C (pH 9.0). The HA microspheres with a diameter of 45–90 μm were hand packed into a steel column (4.6 mm × 80 mm) and used to separate a binary protein mixture of bovine serum albumin (BSA) and lysozyme. HA microspheres, with a diameter <45 μm, were used for separating a protein mixture of BSA, myoglobin, and lysozyme. These microspheres had a diameter that was 20–30 times larger than commercial HA column packing spherical particles, 2–3 μm, but these microspheres had a six times larger surface area and a more uniform spherical shape. These advantages compensated for their larger size and the separation results were comparable to those commercially available HA columns in the separation of the proteins studied. These unique HA microspheres, made from microspheres of a borate glass, are considered to be useful as packing materials for protein separation in chromatography. |
| type | Article - Journal |
| type.DCMIType | text |
| type.status | Postprint |
| relation.isPartOf | Journal of Materials Science |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. |
| rights | Pre-print: author can archive; Post-print: author can archive; |
| rights.URI | |
| rights.URI | |
| identifier.persist.URI | |
| date.available | 2008-12-22T22:09:57Z |