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| Title: | Enzyme linked immunoassay analysis (ELISA) of Atrazine in raw and finished drinking water |
| Author (s): | Jiang, H. Graziano, N. Roberson, A. McGuire, M. |
| Department/Lab Affiliations: | Civil, Architectural & Environmental Engineering Environmental Research Center |
| Keywords: | ELISA Enzyme-Linked immunosorbent assay validation |
| Subject Terms: | Atrazine |
| Issue Date: | 2006 |
| Publisher: | Mary Ann Lieberty |
| Citation: | Jiang, H., Adams, C., Graziano, N., Roberson, A., McGuire, M. (2006) “Enzyme Linked Immunoassay Analysis (ELISA) of Atrazine in Raw and Finished Drinking Water,” Environmental Engineering Science, 20, 503-515. |
| Abstract: | Enzyme-linked immunosorbent (ELISA) tests are commonly used to measure atrazine concentrations in raw and treated drinking water for monitoring variations in atrazine concentrations, process control (e.g., powdered activated carbon dosing), and other purposes. ELISA has also been considered for compliance monitoring. The purpose of this study was to evaluate the accuracy of common commercial atrazine ELISA tests in a variety of surface waters. Seventeen raw and finished drinking water samples from various water supplies were analyzed using three different commercial ELISA test kits and using gas chromatography/ mass spectrometry (GC/MS) with solid-phase extraction. Each sample was analyzed in duplicate, as received, and also with 1-µg/L atrazine spike to test recoveries. The results showed that the commercial ELISA kits provided reproducible results though their accuracy varied between waters. Recoveries in raw and finished waters for the Abraxis™, Beacon™, and SDI™ kits averaged 129, 132, and 113% in raw water, respectively, and 113, 141, and 112% in finished water, respectively. Overall recoveries of a 1- µg/L spike ranged from 71–222, 69–294, and 58–229% for the three kits, respectively. While most biases observed were positive, negative biases were also observed, which is significant in that the ELISA results underestimated the true atrazine concentrations present. |
| Type: | Article - Journal text |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder FULL COPYRIGHT INFORMATION: |
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| title | Enzyme linked immunoassay analysis (ELISA) of Atrazine in raw and finished drinking water |
| contributor | Adams, Craig D. |
| contributor.author | Jiang, H. |
| contributor.author | Graziano, N. |
| contributor.author | Roberson, A. |
| contributor.author | McGuire, M. |
| contributor.deptlab | Civil, Architectural & Environmental Engineering |
| contributor.deptlab | Environmental Research Center |
| contributor.sponsor | Awwa Research Foundation |
| subject | ELISA |
| subject | Enzyme-Linked immunosorbent assay |
| subject | validation |
| subject.LCSH | Atrazine |
| date.issued | 2006 |
| publisher | Mary Ann Lieberty |
| identifier.citation | Jiang, H., Adams, C., Graziano, N., Roberson, A., McGuire, M. (2006) “Enzyme Linked Immunoassay Analysis (ELISA) of Atrazine in Raw and Finished Drinking Water,” Environmental Engineering Science, 20, 503-515. |
| description.abstract | Enzyme-linked immunosorbent (ELISA) tests are commonly used to measure atrazine concentrations in raw and treated drinking water for monitoring variations in atrazine concentrations, process control (e.g., powdered activated carbon dosing), and other purposes. ELISA has also been considered for compliance monitoring. The purpose of this study was to evaluate the accuracy of common commercial atrazine ELISA tests in a variety of surface waters. Seventeen raw and finished drinking water samples from various water supplies were analyzed using three different commercial ELISA test kits and using gas chromatography/ mass spectrometry (GC/MS) with solid-phase extraction. Each sample was analyzed in duplicate, as received, and also with 1-µg/L atrazine spike to test recoveries. The results showed that the commercial ELISA kits provided reproducible results though their accuracy varied between waters. Recoveries in raw and finished waters for the Abraxis™, Beacon™, and SDI™ kits averaged 129, 132, and 113% in raw water, respectively, and 113, 141, and 112% in finished water, respectively. Overall recoveries of a 1- µg/L spike ranged from 71–222, 69–294, and 58–229% for the three kits, respectively. While most biases observed were positive, negative biases were also observed, which is significant in that the ELISA results underestimated the true atrazine concentrations present. |
| type | Article - Journal |
| type.DCMIType | text |
| type.status | Final version |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder |
| rights.URI | |
| date.accessioned | 2007-04-11T17:00:48Z |
| date.available | 2007-12-17T20:46:12Z |
| identifier.persist.URI |