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| Title: | Engineering bacteria for production of rhamnolipid as an agent for enhanced oil recovery |
| Author (s): | Wang, Qinhong Fang, Xiangdong Bai, Baojun Liang, Xiaolin Shuler, Patrick J. Goddard III, William A. Tang, Yongchun |
| Department/Lab Affiliations: | Energy Research and Development Center Geological Sciences & Engineering |
| Keywords: | biosurfactant chromosomal insertion enhanced oil recovery interfacial tension rhamnolipid transposome |
| Issue Date: | 2007-05-07 |
| Publisher: | John Wiley & Sons |
| Citation: | Wang, Qinhong., Fang, Xiangdong., Bai, Baojun., Liang, Xiaolin., Shuler, Patrick J., Goddard III, William A., and Tang, Yongchun. "Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced Oil Recovery.", Biotechnology and Bioengineering, vol. 98, no. 4, 2007. |
| Abstract: | Rhamnolipid as a potent natural biosurfactant has a wide range of potential applications, including enhanced oil recovery (EOR), biodegradation, and bioremediation. Rhamnolipid is composed of rhamnose sugar molecule and beta-hydroxyalkanoic acid. The rhamnosyltransferase 1 complex (RhlAB) is the key enzyme responsible for transferring the rhamnose moiety to the beta-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid. Through transposome-mediated chromosome integration, the RhlAB gene was inserted into the chromosome of the Pseudomonas aeruginosa PAO1-rhlA(-) and Escherichia coli BL21 (DE3), neither of which could produce rhamnolipid. After chromosome integration of the RhlAB gene, the constitute strains P. aeruginosa PEER02 and E. coli TnERAB did produce rhamnolipid. The HPLC/MS spectrum showed that the structure of purified rhamnolipid from P. aeruginosa PEER02 was similar to that from other P. aeruginosa strains, but with different percentage for each of the several congeners. The main congener (near 60%) of purified rhamnolipid from E. coli TnERAB was 3-(3-hydroxydecanoyloxy) decanoate (C(10)-C(10)) with mono-rhamnose. The surfactant performance of rhamnolipid was evaluated by measurement of interfacial tension (IFT) and oil recovery via sand-pack flooding tests. As expected, pH and salt concentration of the rhamnolipid solution significantly affected the IFT properties. With just 250 mg/L rhamnolipid (from P. aeruginosa PEER02 with soybean oil as substrate) in citrate-Na(2)HPO(4), pH 5, 2% NaCl, 42% of oil otherwise trapped was recovered from a sand pack. This result suggests rhamnolipid might be considered for EOR applications. |
| Type: | Article - Journal text |
| In Title: | Biotechnology and Bioengineering |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. Pre-print: author can archive; Post-print: author can archive; FULL COPYRIGHT INFORMATION: |
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| title | Engineering bacteria for production of rhamnolipid as an agent for enhanced oil recovery |
| contributor.author | Wang, Qinhong |
| contributor.author | Fang, Xiangdong |
| contributor.author | Bai, Baojun |
| contributor.author | Liang, Xiaolin |
| contributor.author | Shuler, Patrick J. |
| contributor.author | Goddard III, William A. |
| contributor.author | Tang, Yongchun |
| contributor.deptlab | Energy Research and Development Center |
| contributor.deptlab | Geological Sciences & Engineering |
| subject | biosurfactant |
| subject | chromosomal insertion |
| subject | enhanced oil recovery |
| subject | interfacial tension |
| subject | rhamnolipid |
| subject | transposome |
| date.issued | 2007-05-07 |
| publisher | John Wiley & Sons |
| identifier.citation | Wang, Qinhong., Fang, Xiangdong., Bai, Baojun., Liang, Xiaolin., Shuler, Patrick J., Goddard III, William A., and Tang, Yongchun. "Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced Oil Recovery.", Biotechnology and Bioengineering, vol. 98, no. 4, 2007. |
| identifier.pub.URI | |
| description.abstract | Rhamnolipid as a potent natural biosurfactant has a wide range of potential applications, including enhanced oil recovery (EOR), biodegradation, and bioremediation. Rhamnolipid is composed of rhamnose sugar molecule and beta-hydroxyalkanoic acid. The rhamnosyltransferase 1 complex (RhlAB) is the key enzyme responsible for transferring the rhamnose moiety to the beta-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid. Through transposome-mediated chromosome integration, the RhlAB gene was inserted into the chromosome of the Pseudomonas aeruginosa PAO1-rhlA(-) and Escherichia coli BL21 (DE3), neither of which could produce rhamnolipid. After chromosome integration of the RhlAB gene, the constitute strains P. aeruginosa PEER02 and E. coli TnERAB did produce rhamnolipid. The HPLC/MS spectrum showed that the structure of purified rhamnolipid from P. aeruginosa PEER02 was similar to that from other P. aeruginosa strains, but with different percentage for each of the several congeners. The main congener (near 60%) of purified rhamnolipid from E. coli TnERAB was 3-(3-hydroxydecanoyloxy) decanoate (C(10)-C(10)) with mono-rhamnose. The surfactant performance of rhamnolipid was evaluated by measurement of interfacial tension (IFT) and oil recovery via sand-pack flooding tests. As expected, pH and salt concentration of the rhamnolipid solution significantly affected the IFT properties. With just 250 mg/L rhamnolipid (from P. aeruginosa PEER02 with soybean oil as substrate) in citrate-Na(2)HPO(4), pH 5, 2% NaCl, 42% of oil otherwise trapped was recovered from a sand pack. This result suggests rhamnolipid might be considered for EOR applications. |
| type | Article - Journal |
| type.DCMIType | text |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. |
| rights | Pre-print: author can archive; Post-print: author can archive; |
| rights.URI | |
| rights.URI | |
| relation.isPartOf | Biotechnology and Bioengineering |
| date.available | 2008-07-29T20:48:22Z |
| identifier.persist.URI |