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| Title: | Effects of selenocystine on lead-exposed chinese hamster ovary (CHO) and PC-12 cells |
| Author (s): | Aykin-Burns, N. Ercal, Nuran |
| Department/Lab Affiliations: | Chemistry Environmental Research Center |
| Keywords: | Lead toxicity Reactive oxygen species Selenocysteine Thiol antioxidants |
| Issue Date: | 2006 |
| Publisher: | Elsevier |
| Citation: | Aykin-Burns, N., and Ercal, N. “Effects of Selenocystine on Lead-exposed Chinese Hamster Ovary (CHO) and PC-12 Cells” Toxicol Appl Pharmacol, 2006 |
| Abstract: | Lead is a pervasive environmental toxin that affects multiple organ systems, including the nervous, renal, reproductive, and hematological systems. Even though it is probably the most studied toxic metal, some of the symptoms of lead toxicity still cannot be explained by known molecular mechanisms. Therefore, lead-induced oxidative stress has recently started to gain attention. This in vitro study confirms the existence of oxidative stress due to lead exposure. Administration of lead acetate (PbA) to cultures of Chinese hamster ovary cells (CHO) had a concentration-dependent inhibitory effect on colony formation and cell proliferation. This inhibition was eliminated by 5 μM selenocystine (SeCys). In order to evaluate the nature of SeCys's effect, we measured glutathione (GSH), its oxidized form glutathione disulfide (GSSG), malondialdehyde (MDA), catalase, and GSH peroxidase (GPx) activities in lead-exposed CHO cells both in the presence and absence of SeCys. Increases in MDA, catalase, and GPx activities were observed in cultures that received only PbA, but supplementation with SeCys returned these measures to pretreatment levels. The ratio of GSH to GSSG increased in lead-exposed cells incubated in SeCys-enhanced media but declined in cultures treated with PbA only. In order to determine whether SeCys also reverses lead-induced neurotoxicity, a neuronal cell line, PC-12 cells, was used. Lead's inhibition on neurite formation was significantly eliminated by SeCys in PC-12 cells. Our results suggest that SeCys can confer protection against lead-induced toxicity in CHO cells and neurotoxicity in PC-12 cells. |
| Type: | Article - Journal text |
| In Title: | Toxicology and Applied Pharmacology |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. FULL COPYRIGHT INFORMATION: |
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| title | Effects of selenocystine on lead-exposed chinese hamster ovary (CHO) and PC-12 cells |
| contributor.author | Aykin-Burns, N. |
| contributor.author | Ercal, Nuran |
| contributor.deptlab | Chemistry |
| contributor.deptlab | Environmental Research Center |
| contributor.sponsor | National Institute of Environmental Health |
| subject | Lead toxicity |
| subject | Reactive oxygen species |
| subject | Selenocysteine |
| subject | Thiol antioxidants |
| date.issued | 2006 |
| publisher | Elsevier |
| identifier.citation | Aykin-Burns, N., and Ercal, N. “Effects of Selenocystine on Lead-exposed Chinese Hamster Ovary (CHO) and PC-12 Cells” Toxicol Appl Pharmacol, 2006 |
| identifier.pub.URI | |
| description.abstract | Lead is a pervasive environmental toxin that affects multiple organ systems, including the nervous, renal, reproductive, and hematological systems. Even though it is probably the most studied toxic metal, some of the symptoms of lead toxicity still cannot be explained by known molecular mechanisms. Therefore, lead-induced oxidative stress has recently started to gain attention. This in vitro study confirms the existence of oxidative stress due to lead exposure. Administration of lead acetate (PbA) to cultures of Chinese hamster ovary cells (CHO) had a concentration-dependent inhibitory effect on colony formation and cell proliferation. This inhibition was eliminated by 5 μM selenocystine (SeCys). In order to evaluate the nature of SeCys's effect, we measured glutathione (GSH), its oxidized form glutathione disulfide (GSSG), malondialdehyde (MDA), catalase, and GSH peroxidase (GPx) activities in lead-exposed CHO cells both in the presence and absence of SeCys. Increases in MDA, catalase, and GPx activities were observed in cultures that received only PbA, but supplementation with SeCys returned these measures to pretreatment levels. The ratio of GSH to GSSG increased in lead-exposed cells incubated in SeCys-enhanced media but declined in cultures treated with PbA only. In order to determine whether SeCys also reverses lead-induced neurotoxicity, a neuronal cell line, PC-12 cells, was used. Lead's inhibition on neurite formation was significantly eliminated by SeCys in PC-12 cells. Our results suggest that SeCys can confer protection against lead-induced toxicity in CHO cells and neurotoxicity in PC-12 cells. |
| type | Article - Journal |
| type.DCMIType | text |
| type.status | Final version |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. |
| rights.URI | |
| relation.isPartOf | Toxicology and Applied Pharmacology |
| date.accessioned | 2007-04-11T17:00:48Z |
| date.available | 2007-12-17T20:44:57Z |
| identifier.persist.URI |