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| Title: | Distribution of cells between solid/liquid and liquid/liquid interfaces |
| Author (s): | Bermudez, Ondrea Forciniti, Daniel |
| Department/Lab Affiliations: | Center for Environmental Science and Technology (CEST) Chemical & Biological Engineering |
| Keywords: | aqueous two-phase systems bacterial growth electron microscopy hydroxyapatite discs |
| Issue Date: | 2004 |
| Publisher: | American Chemical Society |
| Citation: | Bermudez, Ondrea., and Forciniti, Daniel. "Distribution of Cells Between Solid/Liquid and Liquid/Liquid Interfaces." Biotechnology Progress, vol. 20, no. 1, 289 -298, 2004. |
| Abstract: | The use of aqueous two-phase systems (ATPSs) and each system's individual phase-forming species to prevent Streptococcus sanguis attachment onto hydroxyapatite discs was explored. The strategy that we followed was to attach the cells to a solid surface in the presence of an additional interface. Conditions under which, simultaneously, the phase-forming species form two phases and the cells proliferate were identified. Growth curves were constructed in the presence of various polymers and salts commonly used to prepare ATPSs. Several aqueous two-phase systems were selected such that bacterial growth was comparable to that observed in pure medium. Cells were allowed to attach to hydroxyapatite discs for 7 days in the presence of varying concentrations of media, media with polymer, media with salt, and media with ATPS. Streptococcus sanguis attachment to the disks was evaluated by scanning electron microscopy. The addition of a PEG/Na2SO4 ATPS to high concentrations of yeast-tryptone (YT) media (>65%) and of a PEG/MgSO4 ATPS to nutrient-limited media reduces surface coverage of S. sanguis to less than 10%. Comparison of the attachment levels for the systems containing PEG/Na2SO4 to media containing the individual phase-forming species and to the YT reference systems indicated that nutrient availability did not affect attachment. |
| Type: | Article - Journal text |
| In Title: | Biotechnology Progress |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. FULL COPYRIGHT INFORMATION: |
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| title | Distribution of cells between solid/liquid and liquid/liquid interfaces |
| contributor.author | Bermudez, Ondrea |
| contributor.author | Forciniti, Daniel |
| contributor.deptlab | Center for Environmental Science and Technology (CEST) |
| contributor.deptlab | Chemical & Biological Engineering |
| subject | aqueous two-phase systems |
| subject | bacterial growth |
| subject | electron microscopy |
| subject | hydroxyapatite discs |
| date.issued | 2004 |
| publisher | American Chemical Society |
| identifier.citation | Bermudez, Ondrea., and Forciniti, Daniel. "Distribution of Cells Between Solid/Liquid and Liquid/Liquid Interfaces." Biotechnology Progress, vol. 20, no. 1, 289 -298, 2004. |
| identifier.pub.URI | |
| description.abstract | The use of aqueous two-phase systems (ATPSs) and each system's individual phase-forming species to prevent Streptococcus sanguis attachment onto hydroxyapatite discs was explored. The strategy that we followed was to attach the cells to a solid surface in the presence of an additional interface. Conditions under which, simultaneously, the phase-forming species form two phases and the cells proliferate were identified. Growth curves were constructed in the presence of various polymers and salts commonly used to prepare ATPSs. Several aqueous two-phase systems were selected such that bacterial growth was comparable to that observed in pure medium. Cells were allowed to attach to hydroxyapatite discs for 7 days in the presence of varying concentrations of media, media with polymer, media with salt, and media with ATPS. Streptococcus sanguis attachment to the disks was evaluated by scanning electron microscopy. The addition of a PEG/Na2SO4 ATPS to high concentrations of yeast-tryptone (YT) media (>65%) and of a PEG/MgSO4 ATPS to nutrient-limited media reduces surface coverage of S. sanguis to less than 10%. Comparison of the attachment levels for the systems containing PEG/Na2SO4 to media containing the individual phase-forming species and to the YT reference systems indicated that nutrient availability did not affect attachment. |
| type | Article - Journal |
| type.DCMIType | text |
| type.status | Final version |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. |
| rights.URI | |
| relation.isPartOf | Biotechnology Progress |
| date.accessioned | 2008-05-20T19:40:36Z |
| date.available | 2008-05-30T19:24:34Z |
| identifier.persist.URI |