Scholars' Mine
Missouri S&T
Research Repository
Curtis Laws Wilson Library
400 W. 14th Street
Rolla, MO 65409-0060
scholarsmine@mst.edu
| Title: | Aggregation and denaturation of antibodies: a capillary electrophoresis, dynamic light scattering, and aqueous two-phase partitioning study |
| Author (s): | Bermudez, Ondrea Forciniti, Daniel |
| Department/Lab Affiliations: | Center for Environmental Science and Technology (CEST) Chemical & Biological Engineering |
| Keywords: | Aggregation Aqueous two-phase systems Denaturation Dynamic light scattering Immunoglobulin G Partitioning |
| Issue Date: | 2004-07-25 |
| Publisher: | Elsevier |
| Citation: | Bermudez, Ondrea., and Forciniti, Daniel. "Aggregation and Denaturation of Antibodies: A Capillary Electrophoresis, Dynamic Light Scattering, and Aqueous Two-Phase Partitioning Study." Journal of Chromatography B, vol. 807, no. 1, pg 17-24, 2004. |
| Abstract: | Protein denaturation and aggregation are well-known problems in the pharmaceutical industry. As the protein aggregates, it loses its biological activity and creates problems in its administration to patients. In this paper, we explore the use of aqueous two-phase systems, capillary zone electrophoresis, and dynamic light scattering for the monitoring of protein denaturation and aggregation. Our studies focus on human IgG and HSA. Capillary zone electrophoresis was used to monitor changes in the charge to size ratio of the proteins upon denaturation and dynamic light scattering was used to detect the presence of any aggregates and to monitor the size of the proteins. The information obtained from aqueous two-phase partitioning is similar to that obtained from capillary zone electrophoresis. The simplicity of aqueous two-phase system and its low cost (compared to the other analytical techniques) suggest that it can be routinely used for the quality control of some pharmaceutical preparations. |
| Type: | Article - Journal text |
| In Title: | Journal of Chromatography B |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. FULL COPYRIGHT INFORMATION: |
| Publisher URL: | |
| Link to this page: |
| title | Aggregation and denaturation of antibodies: a capillary electrophoresis, dynamic light scattering, and aqueous two-phase partitioning study |
| contributor.author | Bermudez, Ondrea |
| contributor.author | Forciniti, Daniel |
| contributor.deptlab | Center for Environmental Science and Technology (CEST) |
| contributor.deptlab | Chemical & Biological Engineering |
| subject | Aggregation |
| subject | Aqueous two-phase systems |
| subject | Denaturation |
| subject | Dynamic light scattering |
| subject | Immunoglobulin G |
| subject | Partitioning |
| date.issued | 2004-07-25 |
| publisher | Elsevier |
| identifier.citation | Bermudez, Ondrea., and Forciniti, Daniel. "Aggregation and Denaturation of Antibodies: A Capillary Electrophoresis, Dynamic Light Scattering, and Aqueous Two-Phase Partitioning Study." Journal of Chromatography B, vol. 807, no. 1, pg 17-24, 2004. |
| identifier.pub.URI | |
| description.abstract | Protein denaturation and aggregation are well-known problems in the pharmaceutical industry. As the protein aggregates, it loses its biological activity and creates problems in its administration to patients. In this paper, we explore the use of aqueous two-phase systems, capillary zone electrophoresis, and dynamic light scattering for the monitoring of protein denaturation and aggregation. Our studies focus on human IgG and HSA. Capillary zone electrophoresis was used to monitor changes in the charge to size ratio of the proteins upon denaturation and dynamic light scattering was used to detect the presence of any aggregates and to monitor the size of the proteins. The information obtained from aqueous two-phase partitioning is similar to that obtained from capillary zone electrophoresis. The simplicity of aqueous two-phase system and its low cost (compared to the other analytical techniques) suggest that it can be routinely used for the quality control of some pharmaceutical preparations. |
| type | Article - Journal |
| type.DCMIType | text |
| type.status | Final version |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. |
| rights.URI | |
| relation.isPartOf | Journal of Chromatography B |
| date.accessioned | 2007-04-11T17:00:48Z |
| date.available | 2008-05-30T19:19:46Z |
| identifier.persist.URI |