Scholars' Mine
Missouri S&T
Research Repository
Curtis Laws Wilson Library
400 W. 14th Street
Rolla, MO 65409-0060
scholarsmine@mst.edu
| Title: | Accuracy and interferences for enzyme-linked Immunoassay tests for Atrazine |
| Author (s): | Adams, Craig D. Jiang, H. McGuire, M. Graziano, N. Roberson, A . Frey, M. |
| Department/Lab Affiliations: | Civil, Architectural & Environmental Engineering Environmental Research Center |
| Keywords: | Accuracy Enzyme-linked Immunoassay Test |
| Subject Terms: | Atrazine |
| Issue Date: | 2004 |
| Publisher: | American Water Works Association |
| Citation: | Adams, C., Jiang, H., Graziano, N., Roberson, A., McGuire, M.,Frey, M. (2004) “Accuracy and Interferences for Enzyme-Linked Immunoassay Tests for Atrazine,” J. American Water Works Association, 96:12, 126-139. |
| Abstract: | Atrazine is one of the most commonly used herbicides in the United States, therefore it is a regulatory concern and is extensively monitored in the environment, especially in drinking water. Because of the higher costs of gas chromatography (GC) methods, utility managers and regulators are considering the increased use of enzyme-linked immunosorbent assay (ELISA) methods for monitoring atrazine. An ELISA test kit was recently approved by the US Environmental Protection Agency. The research in this article points out potential biases and inaccuracies that may occur with such test kits. ELISA test kits for atrazine are typically precise (repeatable) but often have a tendency for a positive bias from unknown interferences. Negative biases are also observed in some cases, which suggest a lower atrazine concentration than is actually present. The results of this research can help utilities and regulators to better determine the best use of ELISA versus GC methods for analyzing atrazine with respect to monitoring and/or process control. |
| Type: | Article - Journal text |
| Copyright Notice: | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. FULL COPYRIGHT INFORMATION: |
| Publisher URL: | |
| Link to this page: |
| title | Accuracy and interferences for enzyme-linked Immunoassay tests for Atrazine |
| contributor.author | Adams, Craig D. |
| contributor.author | Jiang, H. |
| contributor.author | McGuire, M. |
| contributor.author | Graziano, N. |
| contributor.author | Roberson, A . |
| contributor.author | Frey, M. |
| contributor.deptlab | Civil, Architectural & Environmental Engineering |
| contributor.deptlab | Environmental Research Center |
| contributor.sponsor | Awwa Research Foundation |
| subject | Accuracy |
| subject | Enzyme-linked |
| subject | Immunoassay Test |
| subject.LCSH | Atrazine |
| date.issued | 2004 |
| publisher | American Water Works Association |
| identifier.citation | Adams, C., Jiang, H., Graziano, N., Roberson, A., McGuire, M.,Frey, M. (2004) “Accuracy and Interferences for Enzyme-Linked Immunoassay Tests for Atrazine,” J. American Water Works Association, 96:12, 126-139. |
| identifier.pub.URI | |
| description.abstract | Atrazine is one of the most commonly used herbicides in the United States, therefore it is a regulatory concern and is extensively monitored in the environment, especially in drinking water. Because of the higher costs of gas chromatography (GC) methods, utility managers and regulators are considering the increased use of enzyme-linked immunosorbent assay (ELISA) methods for monitoring atrazine. An ELISA test kit was recently approved by the US Environmental Protection Agency. The research in this article points out potential biases and inaccuracies that may occur with such test kits. ELISA test kits for atrazine are typically precise (repeatable) but often have a tendency for a positive bias from unknown interferences. Negative biases are also observed in some cases, which suggest a lower atrazine concentration than is actually present. The results of this research can help utilities and regulators to better determine the best use of ELISA versus GC methods for analyzing atrazine with respect to monitoring and/or process control. |
| type | Article - Journal |
| type.DCMIType | text |
| type.status | Final version |
| rights | This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. |
| rights.URI | |
| date.accessioned | 2007-04-11T17:00:48Z |
| date.available | 2007-12-12T23:25:29Z |
| identifier.persist.URI |