Simultaneous Determination of Polyamines and Catecholamines in PC-12 Tumor Cell Extracts by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
A method to detect polyamines and catecholamines in PC-12 tumor cell extracts by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is described for the first time. Both derivatization conditions and buffer concentrations and pH were optimized. under optimized conditions the polyamines (putresine, spermine, spermidine) and catecholamines (dopamine, norepinephrine, epinephrine, serotonin) were derivatized with fluorescein isothiocyanate and separated at 25 kV in a fused-silica capillary (50 m ID×40 cm) with 0.1 M borate, pH 9.0, in less than 18 min. The influence of running buffer conditions, such as buffer pH and concentrations, were also investigated. Linearity of the analytes ranged from 0.05 to 1.0 mol/L, and the detection limit (S/N = 3 ) ranged from 0.03 to 2.50 nmol/L. The concentrations of polyamines and catecholamines in PC-12 tumor cell extracts were determined with this method.
M. Du et al., "Simultaneous Determination of Polyamines and Catecholamines in PC-12 Tumor Cell Extracts by Capillary Electrophoresis with Laser-Induced Fluorescence Detection," Electrophresis, vol. 25, no. 10-11, pp. 1496-1502, John Wiley & Sons, Jun 2004.
The definitive version is available at http://dx.doi.org/10.1002/elps.200405896
Mechanical and Aerospace Engineering
Keywords and Phrases
Adrenalin; Boric Acid; Catecholamine Derivative; Cell Extract; Dopamine; Fluorescein Isothiocyanate; Noradrenalin; Polyamine Derivative; Putrescine; Serotonin; Silicon Dioxide; Spermidine; Spermine; Animal Cell; Capillary Electrophoresis; Concentration (Parameters); Controlled Study; Derivatization; Fluorescence Analysis; Linear System; Nonhuman; PH; Process Optimization; Quantitative Analysis; Rat; Reproducibility; Sensitivity Analysis; Separation Technique; Tumor Cell; Animals; Catecholamines; Cell Extracts; Electrophoresis, Capillary; Hydrogen-Ion Concentration; PC12 Cells; Polyamines; Rats
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International Standard Serial Number (ISSN)
Article - Journal
© 2004 John Wiley & Sons, All rights reserved.