In Vitro Evaluation of Cytotoxicity of Silver-containing Borate Bioactive Glass
Gilbert, Jeremy L.
The cytotoxicity of silver-containing borate bioactive glass was evaluated in vitro from the response of osteoblastic and fibroblastic cells in media containing the dissolution products of the glass. Glass frits containing 0−2 weight percent (wt %) Ag were prepared by a conventional melting and quenching process. the amount of Ag dissolved from the glass into a simulated body fluid (SBF), measured using atomic emission spectroscopy, increased rapidly within the first 48 h, but slowed considerably at longer times. Structural and microchemical analysis showed that the formation of a hydroxyapatite-like layer on the glass surface within 14 days of immersion in the SBF. the response of MC3T3-E1 and L929 cells to the dissolution products of the glass was evaluated using SEM observation of cell morphology, and assays of MTT hydrolysis, lactate dehydrogenase release, and alkaline phosphatase activity after incubation for up to 48 h. Cytotoxic effects were found for the borate glass containing 2 wt % Ag, but not for 0.75 and 1 wt % Ag. This borate glass containing up to ∼1 wt % Ag could provide a coating material for bacterial inhibition and enhanced bioactivity of orthopaedic implant materials such as titanium. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.
S. Luo et al., "In Vitro Evaluation of Cytotoxicity of Silver-containing Borate Bioactive Glass," Journal of Biomedical Materials Research - Part B: Applied Biomaterials, Wiley-Blackwell, Jan 2010.
The definitive version is available at http://dx.doi.org/10.1002/jbm.b.31735
Materials Science and Engineering
Keywords and Phrases
Silver-Containing Borate Glass; Cytotoxicity; Fibroblast; Osteoblast
Article - Journal
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