Effect of Immersion Medium on the Degradation and Conversion of Silicate (13-93) Bioactive Glass Scaffolds
Simulated body fluid (SBF) has been widely used to assess the bioactivity of materials in vitro but a limitation is that it lacks the proteins present in human blood serum. In the present study, the degradation of silicate 13-93 bioactive glass and its conversion to hydroxyapatite (HA) in Dulbecco's modified Eagle's medium (DMEM), a widely used cell culture medium, were studied and compared with its behavior in SBF. The degradation of the glass, determined from the release of Si or K ions from the glass into the medium, showed little difference between DMEM and SBF but the conversion to HA was slower in DMEM. HA was detected on the surface of the glass within 4 weeks of immersion in SBF but within 6 weeks of immersion in DMEM. The results indicate that DMEM could be used as an alternative to SBF in evaluating the bioactivity of materials.
Y. Gu et al., "Effect of Immersion Medium on the Degradation and Conversion of Silicate (13-93) Bioactive Glass Scaffolds," Ceramic Transactions, vol. 254, pp. 73-83, American Ceramic Society, Jan 2015.
The definitive version is available at http://dx.doi.org/10.1002/9781119190134.ch8
Next Generation Biomaterials and Surface Properties of Biomaterials Symposia - Materials Science and Technology Conference (2014: Oct 12-16, Pittsburgh, PA)
Materials Science and Engineering
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