Title

Albumin Conformational Change and Aggregation Induced by Nanostructured Apatites

Abstract

Biomaterials with nanostructured surfaces influence cellular response in a significantly different, and often beneficial, manner compared to materials with coarser features. Hydroxyapatite [HA, Ca10(PO4)6(OH)2] and strontium-apatite [Sr10(PO4)6(OH)2] microspheres that present nanotopographies similar to biological apatites were incubated in albumin solutions, at physiological conditions (40 mg ml-1; 37 °C), for up to 72 h. Electronic and vibrational circular dichroism spectroscopies revealed spectral signatures characteristic of stacked β-sheet regions in higher ordered structures (e.g., fibrils). The presence of stacked β-sheets was further evidenced by thioflavin T staining. The sequestration of interfacial Ca atoms by pyrophosphate ions (P2O7 4-), prior to albumin adsorption, prevented stacked β-sheet formation on hydroxyapatite. These results suggest that the charge and/or spatial arrangement of Ca atoms direct stacked β-sheet formation during bovine serum albumin adsorption. Stacked β-sheet spectral features were also observed after incubating HA in fetal bovine serum, highlighting that this phenomena could direct cellular response to these biomaterials in vivo.

Department(s)

Materials Science and Engineering

International Standard Serial Number (ISSN)

19348630

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2017 American Institute of Physics (AIP), All rights reserved.


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