Title

Preparation and Bioactive Characteristics of Porous Borate Glass Substrates

Abstract

Whereas silicate-based bioactive glasses and glass-ceramics have been widely investigates for bone repair or as scaffolds for cell-based bone tissue engineering, recent data have demonstrated that silica-free borate glasses also exhibit bioactive behavior. The objectives of this study were to fabricate porous, three-dimensional substrates by in vitro cell culture with human mesenchymal stem cells (hMSCs) and hMSC-derived osteoblasts (hMSC-Obs).Borate glass particles with sizes 212-355 µm were loosely compacted and then sintered at 6000C to form porous disc-shaped substrates (porosity ≈40%).Partial or nearly complete conversion of the glass substrates to a calcium phosphate(Ca-P) material was achieved by soaking the substrates for 1 day or 7 days in a 0.25 molar K2HPO4 solution at 370C and at pH of 9.0. Bone marrow derived hMSC-Obs markedly synthesized alkaline phosphatase, an early osteogenic marker. These data indicate strong bioactive characteristics for the borate glass constructs and the potential use of the constructs for bone tissue engineering.

Department(s)

Materials Science and Engineering

Sponsor(s)

National Institute of Health (U.S.)
University of Illinois at Chicago
University of Illinois at Urbana-Champaign
University of Missouri Research Board
Whitaker Foundation

Document Type

Article - Conference proceedings

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2005 American Ceramic Society, All rights reserved.

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