Masters Theses

Title

Oxidative stress, calcium homeostasis, and altered gene expression in human lung epithelial cells exposed to ZnO nanoparticles

Abstract

"The influence of 20 nm ZnO nanoparticles on oxidative stress, intracellular calcium homeostasis, and gene expression was studied in human bronchial epithelial cells (BEAS-2B). ZnO caused a concentration- and time-dependent cytotoxicity while elevating oxidative stress and causing membrane damage (cellular LDH release). There was a remarkably steep relationship between concentration and toxicity at concentrations from 5 to 10 µg/ml. Exposure to ZnO increased intracellular calcium levels in a concentration- and time-dependent manner. Treatment with the antioxidant N-acetylcysteine prevented cell loss and diminished the increase in intracellular calcium concentration, suggesting oxidative stress mediated cytotoxicity. exposure to a sublethal concentration of ZnO increased the expression of BNIP, PRDX3, PRNP, and TXRND1 genes by at least or above 2.5 fold. These four genes are involved in apoptosis and oxidative stress responses"--Abstract, leaf iv.

Advisor(s)

Huang, Yue-wern

Committee Member(s)

Nam, Paul Ki-souk
Aronstam, Robert

Department(s)

Biological Sciences

Degree Name

M.S. in Environmental Biology

Publisher

Missouri University of Science and Technology

Publication Date

Summer 2009

Pagination

viii, 37 leaves

Note about bibliography

Includes bibliographical references.

Rights

© 2009 Chuan-Chin Huang, All rights reserved.

Document Type

Thesis - Citation

File Type

text

Language

English

Library of Congress Subject Headings

Calcium
Gene expression
Nanoparticles
Oxidative stress
Zinc oxide

Thesis Number

T 9541

Print OCLC #

472433673

Link to Catalog Record

Full-text not available: Request this publication directly from Missouri S&T Library or contact your local library.

http://laurel.lso.missouri.edu/record=b7321548~S5

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