Oxidative stress, calcium homeostasis, and altered gene expression in human lung epithelial cells exposed to ZnO nanoparticles
"The influence of 20 nm ZnO nanoparticles on oxidative stress, intracellular calcium homeostasis, and gene expression was studied in human bronchial epithelial cells (BEAS-2B). ZnO caused a concentration- and time-dependent cytotoxicity while elevating oxidative stress and causing membrane damage (cellular LDH release). There was a remarkably steep relationship between concentration and toxicity at concentrations from 5 to 10 µg/ml. Exposure to ZnO increased intracellular calcium levels in a concentration- and time-dependent manner. Treatment with the antioxidant N-acetylcysteine prevented cell loss and diminished the increase in intracellular calcium concentration, suggesting oxidative stress mediated cytotoxicity. exposure to a sublethal concentration of ZnO increased the expression of BNIP, PRDX3, PRNP, and TXRND1 genes by at least or above 2.5 fold. These four genes are involved in apoptosis and oxidative stress responses"--Abstract, leaf iv.
Nam, Paul Ki-souk
M.S. in Environmental Biology
Missouri University of Science and Technology
viii, 37 leaves
© 2009 Chuan-Chin Huang, All rights reserved.
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Full-text not available: Request this publication directly from Missouri S&T Library or contact your local library.http://laurel.lso.missouri.edu/record=b7321548~S5
Huang, Chuan-Chin, "Oxidative stress, calcium homeostasis, and altered gene expression in human lung epithelial cells exposed to ZnO nanoparticles" (2009). Masters Theses. 93.