Elution of beta-galactosidase from the complex between beta-galactosidase and anti-beta-galactosidase monoclonal antibody

Author

Jai-Woo Chang

Abstract

"The elution of beta-galactosidase (BG) from the betagalactosidase- anti-beta-galactosidase monoclonal antibody (BG-ABGMA) complex has been studied in various conditions. The elution of BG from the BG-ABGMA complex was successfully carried out by introducing a polarity reducing agent at relatively mild pH conditions. In addition to the successful elution, these experiments also validated the previously developed experimental technique of measuring the amount of BG that was adsorbed by the antibody. This amount was the difference between an irnmunosorbent adsorption curve and a control adsorption curve"--Abstract, page ii.

Advisor(s)

Poling, Bruce E.

Committee Member(s)

Liapis, Athanasios I.
Rigler, A. K.
Brown, Roger F.

Department(s)

Chemical and Biochemical Engineering

Degree Name

M.S. in Chemical Engineering

Sponsor(s)

University of Missouri--Rolla. Department of Chemical Engineering
University of Missouri--Rolla. Biochemical Processing Institute

Publisher

University of Missouri--Rolla

Publication Date

Fall 1989

Pagination

viii, 95 pages

Note about bibliography

Includes bibliographical references (pages 45-55).

Rights

© 1989 Jai-Woo Chang, All rights reserved.

Document Type

Thesis - Restricted Access

File Type

text

Language

English

Subject Headings

Beta-galactosidase
Antibodies, Monoclonal
Separation (Technology)
Adsorption

Thesis Number

T 5956

Print OCLC #

21265634

Electronic OCLC #

922647675

Link to Catalog Record

Electronic access to the full-text of this document is restricted to Missouri S&T users. Otherwise, request this publication directly from Missouri S&T Library or contact your local library.

Recommended Citation

Chang, Jai-Woo, "Elution of beta-galactosidase from the complex between beta-galactosidase and anti-beta-galactosidase monoclonal antibody" (1989). Masters Theses. 824.
https://scholarsmine.mst.edu/masters_theses/824