Masters Theses

Title

Enantio-enrichment of alpha-hydroxy acids through immobilized-lipase catalyzed hydrolysis of their esters

Alternative Title

Enantio enrichment of alpha hydroxy acids through immobilized lipase catalyzed hydrolysis of their esters

Author

Samit Kapoor

Abstract

"Alpha hydroxy acids (AHAs) have received wide attention in cosmetic and animal feed-industries. HMB, an important member of the AHA group, is the hydroxy analog of methionine. Methionine is a limiting amino acid for a number of animal species. Unlike free methionine, HMB shows resistance to degradation and superior bioavailability upon supplementation to ruminants and avian species...This study was aimed at development and evaluation of an efficient preparatory scale approach for isolation of AHA enantiomers. The process involved selective hydrolysis of AHA-esters in an aqueous system with immobilized enzymes. The free enzyme catalyzed enzymatic reactions were slow compared to those catalyzed with the immobilized enzymes. Chiral LC-MS and LC results for enantio-enriched HMB and lactic acid showed that enantio-purity of >95% can be readily achieved with lipase immobilized in sodium alginate-gelatin blended beads"--Abstract, leaf iii.

Department(s)

Chemical and Biochemical Engineering

Degree Name

M.S. in Chemical Engineering

Publisher

University of Missouri--Rolla

Publication Date

Fall 2005

Pagination

ix, 60 leaves

Rights

© 2005 Samit Kapoor, All rights reserved.

Document Type

Thesis - Citation

File Type

text

Language

English

Library of Congress Subject Headings

Alpha hydroxy acids
Enantiomers
Lipase
Liquid chromatography

Thesis Number

T 8902

Print OCLC #

72441113

Link to Catalog Record

Full-text not available: Request this publication directly from Missouri S&T Library or contact your local library.

http://laurel.lso.missouri.edu/record=b5664995~S5

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