Enantio-enrichment of alpha-hydroxy acids through immobilized-lipase catalyzed hydrolysis of their esters
Enantio enrichment of alpha hydroxy acids through immobilized lipase catalyzed hydrolysis of their esters
"Alpha hydroxy acids (AHAs) have received wide attention in cosmetic and animal feed-industries. HMB, an important member of the AHA group, is the hydroxy analog of methionine. Methionine is a limiting amino acid for a number of animal species. Unlike free methionine, HMB shows resistance to degradation and superior bioavailability upon supplementation to ruminants and avian species...This study was aimed at development and evaluation of an efficient preparatory scale approach for isolation of AHA enantiomers. The process involved selective hydrolysis of AHA-esters in an aqueous system with immobilized enzymes. The free enzyme catalyzed enzymatic reactions were slow compared to those catalyzed with the immobilized enzymes. Chiral LC-MS and LC results for enantio-enriched HMB and lactic acid showed that enantio-purity of >95% can be readily achieved with lipase immobilized in sodium alginate-gelatin blended beads"--Abstract, leaf iii.
Chemical and Biochemical Engineering
M.S. in Chemical Engineering
University of Missouri--Rolla
ix, 60 leaves
© 2005 Samit Kapoor, All rights reserved.
Thesis - Citation
Library of Congress Subject Headings
Alpha hydroxy acids
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Full-text not available: Request this publication directly from Missouri S&T Library or contact your local library.http://laurel.lso.missouri.edu/record=b5664995~S5
Kapoor, Samit, "Enantio-enrichment of alpha-hydroxy acids through immobilized-lipase catalyzed hydrolysis of their esters" (2005). Masters Theses. 5849.